BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 7290

Title: Structural characterization and oligomerization of PB1-F2, a pro-apoptotic influenza A virus protein   PubMed: 17052982

Deposition date: 2006-09-06 Original release date: 2006-10-31

Authors: Bruns, Karsten; Studtrucker, Nicole; Sharma, Alok; Fossen, Torgils; Mitzner, David; Eissmann, Andre; Tessmer, Uwe; Henklein, Peter; Wray, Victor; Schubert, Ulrich

Citation: Bruns, Karsten; Studtrucker, Nicole; Sharma, Alok; Fossen, Torgils; Mitzner, David; Eissmann, Andre; Tessmer, Uwe; Roder, R.; Henklein, Peter; Wray, Victor; Schubert, Ulrich. "Structural characterization and oligomerization of PB1-F2, a pro-apoptotic influenza A virus protein"  J. Biol. Chem. 282, 353-363 (2006).

Assembly members:
PB(30-70), polymer, 41 residues, 4978 Da.

Natural source:   Common Name: Influenza A virus   Taxonomy ID: 211044   Superkingdom: Viruses   Kingdom: Not applicable   Genus/species: Influenzavirus A Influenza A virus

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
PB(30-70): LEHRNSTRLMGHCQKTMNQV VMPKQIVYWKQWLSLRNPIL V

Data sets:
Data typeCount
1H chemical shifts351

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1PB(30-70)1

Entities:

Entity 1, PB(30-70) 41 residues - 4978 Da.

Central fragment PB(30-70) of the PB1-F2 protein comprising amino acid residues Leu-30 to Val-70

1   LEUGLUHISARGASNSERTHRARGLEUMET
2   GLYHISCYSGLNLYSTHRMETASNGLNVAL
3   VALMETPROLYSGLNILEVALTYRTRPLYS
4   GLNTRPLEUSERLEUARGASNPROILELEU
5   VAL

Samples:

sample_1: PB(30-70) 2 mM; Trifluoroethanol 50 % v/v; water 50 % v/v

conditions_1: ionic strength: 0 mM; pH: 3; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
1D 1Hsample_1not availableconditions_1
2D 1H,1H COSYsample_1not availableconditions_1
2D 1H,1H TOCSYsample_1not availableconditions_1
2D 1H,1H NOESYsample_1not availableconditions_1

Software:

XWIN-NMR v3.1, Bruker -

NMR spectrometers:

  • Bruker DMX 600 MHz

Related Database Links:

BMRB 7258
PDB
GB ABD62790 ABD77805 ABF47964 ABM22200 ABO38063
REF YP_418248
SP A4GCJ5 A4GCL7 A4GCM8 P0C0U1 Q20MH0