BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 7305

Title: R21A Spc-SH3 free   PubMed: 17407569

Deposition date: 2006-09-28 Original release date: 2007-05-04

Authors: van Nuland, Nico; Casares, Salvador; AB, Eiso; Eshuis, Henk; Lopez-Mayorga, Obdulio; Conejero-Lara, Francisco

Citation: Casares, Salvador; AB, Eiso; Eshuis, Henk; Lopez-Mayorga, Obdulio; van Nuland, Nico; Conejero-Lara, Francisco. "The high-resolution NMR structure of the R21A Spc-SH3:P41 complex: Understanding the determinants of binding affinity by comparison with Abl-SH3"  BMC Struct. Biol. 7, .-. (2007).

Assembly members:
R21A Spc-SH3 free, polymer, 62 residues, Formula weight is not available

Natural source:   Common Name: Chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
R21A Spc-SH3 free: MDETGKELVLALYDYQEKSP AEVTMKKGDILTLLNSTNKD WWKVEVNDRQGFVPAAYVKK LD

Data sets:
Data typeCount
1H chemical shifts467

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1R21A Spc-SH3 monomer1

Entities:

Entity 1, R21A Spc-SH3 monomer 62 residues - Formula weight is not available

1   METASPGLUTHRGLYLYSGLULEUVALLEU
2   ALALEUTYRASPTYRGLNGLULYSSERPRO
3   ALAGLUVALTHRMETLYSLYSGLYASPILE
4   LEUTHRLEULEUASNSERTHRASNLYSASP
5   TRPTRPLYSVALGLUVALASNASPARGGLN
6   GLYPHEVALPROALAALATYRVALLYSLYS
7   LEUASP

Samples:

sample_1: R21A Spc-SH3 free 2 mM; H2O 90%; D2O 10%; d5-glycine 20 mM

conditions_1: pH: 3.5; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D TOCSYsample_1not availableconditions_1
2D NOESYsample_1not availableconditions_1

Software:

NMRView v5 -

NMR spectrometers:

  • Bruker DRX 750MHz MHz

Related Database Links:

BMRB 15144 17915
PDB
DBJ BAD52438 BAD93097 BAG57892 BAG62120 BAG72795
EMBL CAA29435 CAA32663 CAA62350 CAB53710 CDQ79062
GB AAA51702 AAA51790 AAA52468 AAB41498 AAB60364
REF NP_001036003 NP_001070022 NP_001090674 NP_001091958 NP_001107628
SP P07751 P16086 P16546 Q13813
TPG DAA24188