BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 7311

Title: Backbone 1H, 13C, and 15N and CB Assignments for Rat Islet Amyloid Polypeptide   PubMed: 17123962

Deposition date: 2006-10-01 Original release date: 2007-05-04

Authors: Williamson, Jessica; Miranker, Andrew

Citation: Williamson, Jessica; Miranker, Andrew. "Direct Detection of Transient alpha-Helical States of Islet Amyloid Polypeptide"  Protein Sci. 16, 110-117 (2007).

Assembly members:
Rat Islet Amyliod Polypeptide, polymer, 38 residues, Formula weight is not available

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Rat Islet Amyliod Polypeptide: KCNTATCATQRLANFLVRSS NNLGPVLPPTNVGSNTYX

Data sets:
Data typeCount
13C chemical shifts111
15N chemical shifts37
1H chemical shifts73

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Rat Islet Amyloid Polypeptide1

Entities:

Entity 1, Rat Islet Amyloid Polypeptide 38 residues - Formula weight is not available

1   LYSCYSASNTHRALATHRCYSALATHRGLN
2   ARGLEUALAASNPHELEUVALARGSERSER
3   ASNASNLEUGLYPROVALLEUPROPROTHR
4   ASNVALGLYSERASNTHRTYRNH2

Samples:

sample_1: Rat Islet Amyliod Polypeptide, [U-13C; U-15N], 1.5 mM; potassium chloride 0.1 mM; potassium phosphate 0.05 mM; deuterium oxide 10%

conditions_1: pH: 5.5; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
CBCANHsample_1not availableconditions_1
CBCA(CO)NNHsample_1not availableconditions_1
HNCOsample_1not availableconditions_1
NOESY-15N-HSQCsample_1not availableconditions_1
TOCSY-15N-HSQCsample_1not availableconditions_1
15N-HSQCsample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Varian Unity-Inova 500 MHz

Related Database Links:

BMRB 16314 20046
PDB
DBJ BAA22051
EMBL CAA37003
GB AAA37874 AAA40730 AAA41359 AAH27527 EDL10633
REF NP_034621 NP_036718 XP_006155144 XP_006155145 XP_006237676
SP P12968 P12969

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts