BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 7313

Title: 1H, 13C and 15N Backbone Resonance Assignments of the Pactolus I domain   PubMed: 17523188

Deposition date: 2006-10-09 Original release date: 2007-06-20

Authors: Sen, Mehmet; Legge, Glen

Citation: Sen, Mehmet; Legge, Glen. "Pactolus I-domain: Functional switching of the Rossmann fold"  Proteins 68, 626-635 (2007).

Assembly members:
Pactolus I-domain, polymer, 230 residues, 25366 Da.

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Pactolus I-domain: GSSHHHHHHGLVPRGSHDNS VDLYFLMGLSGSAQGHLSNV QTLGSDLLKALNEISRSGRI GFGSIVNMTFQHILKLTADS SQFQRELRKQLVSGKLATPK GQLDAVVQVAICLGEIGWRN GTRFLVLVTDNDFHLAKDKT LGTRQNTSDGRCHLDDGMYR SRGEPDYQSVVQLASKLAEN NIQPIFVVPSRMVKTYEKLT TFIPKLTIGELSDDSSNVAQ LIRNAYSKLS

Data sets:
Data typeCount
13C chemical shifts482
15N chemical shifts161
1H chemical shifts161

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1I-domain1

Entities:

Entity 1, I-domain 230 residues - 25366 Da.

This sequence contains hexameric Histidine Tag

1   GLYSERSERHISHISHISHISHISHISGLY
2   LEUVALPROARGGLYSERHISASPASNSER
3   VALASPLEUTYRPHELEUMETGLYLEUSER
4   GLYSERALAGLNGLYHISLEUSERASNVAL
5   GLNTHRLEUGLYSERASPLEULEULYSALA
6   LEUASNGLUILESERARGSERGLYARGILE
7   GLYPHEGLYSERILEVALASNMETTHRPHE
8   GLNHISILELEULYSLEUTHRALAASPSER
9   SERGLNPHEGLNARGGLULEUARGLYSGLN
10   LEUVALSERGLYLYSLEUALATHRPROLYS
11   GLYGLNLEUASPALAVALVALGLNVALALA
12   ILECYSLEUGLYGLUILEGLYTRPARGASN
13   GLYTHRARGPHELEUVALLEUVALTHRASP
14   ASNASPPHEHISLEUALALYSASPLYSTHR
15   LEUGLYTHRARGGLNASNTHRSERASPGLY
16   ARGCYSHISLEUASPASPGLYMETTYRARG
17   SERARGGLYGLUPROASPTYRGLNSERVAL
18   VALGLNLEUALASERLYSLEUALAGLUASN
19   ASNILEGLNPROILEPHEVALVALPROSER
20   ARGMETVALLYSTHRTYRGLULYSLEUTHR
21   THRPHEILEPROLYSLEUTHRILEGLYGLU
22   LEUSERASPASPSERSERASNVALALAGLN
23   LEUILEARGASNALATYRSERLYSLEUSER

Samples:

triple_labelled_sample: Pactolus I-domain, [U-99% 2H; U-99% 15N; U-99% 13C], 0.3 ± 0.01 mM; dTris 10 mM; NaN3 0.05%; D20 5%

double_labelled_sample: Pactolus I-domain, [U-99% 15N; U-99% 13C], 0.3 ± 0.01 mM; dTris 10 mM; NaN3 0.05%; D20 5%

15N_labelled_sample: Pactolus I-domain, [U-99% 15N], 0.3 ± 0.01 mM; dTris 10 mM; NaN3 0.05%; D20 5%

conditions: ionic strength: 10 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HNCAtriple_labelled_samplenot availableconditions
HN(CO)CAtriple_labelled_samplenot availableconditions
HNCBtriple_labelled_samplenot availableconditions
HN(CO)CBnot availablenot availableconditions
HN(CA)COtriple_labelled_samplenot availableconditions
HNCOtriple_labelled_samplenot availableconditions
HSQC-NOESY-HSQCtriple_labelled_samplenot availableconditions
CACB(CO)NHtriple_labelled_samplenot availableconditions
CN-NOESYtriple_labelled_samplenot availableconditions
N-TOCSY15N_labelled_samplenot availableconditions
N-NOESY15N_labelled_samplenot availableconditions
HSQC15N_labelled_samplenot availableconditions

Software:

NMRPipe - Data Processing

NMRView - Data Analysis

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 600 MHz

Related Database Links:

PDB
DBJ BAE23399
GB AAC25502 AAI13143 EDL03670 EDL03671
REF NP_032431 XP_006522988 XP_006522989 XP_006522990 XP_006522991
SP Q3UV74

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts