BMRB Entry 7324
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7324
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Title: NMR solution structure of mouse SelW PubMed: 17928294
Deposition date: 2006-10-26 Original release date: 2006-11-06
Authors: Aachmann, Finn; Fomenko, Dmitri; Soragni, Alice; Gladyshev, Vadim; Dikiy, Alexander
Citation: Aachmann, Finn; Fomenko, Dmitri; Soragni, Alice; Gladyshev, Vadim; Dikiy, Alexander. "Solution structure of selenoprotein W and NMR analysis of its interaction with 14-3-3 proteins." J. Biol. Chem. 282, 37036-37044 (2007).
Assembly members:
SelW, polymer, 96 residues, 10463 Da.
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
SelW: MALAVRVVYSGACGYKPKYL
QLKEKLEHEFPGCLDICGEG
TPQVTGFFEVTVAGKLVHSK
KRGDGYVDTESKFRKLVTAI
KAALAQCQLEHHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 360 |
| 15N chemical shifts | 95 |
| 1H chemical shifts | 639 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SelW | 1 |
Entities:
Entity 1, SelW 96 residues - 10463 Da.
SELENOCYSTEINE (CSE) HAS BEEN MUTATED TO CYS. Amino acid 89-96 His-tag, cloning artifact.
| 1 | MET | ALA | LEU | ALA | VAL | ARG | VAL | VAL | TYR | SER | ||||
| 2 | GLY | ALA | CYS | GLY | TYR | LYS | PRO | LYS | TYR | LEU | ||||
| 3 | GLN | LEU | LYS | GLU | LYS | LEU | GLU | HIS | GLU | PHE | ||||
| 4 | PRO | GLY | CYS | LEU | ASP | ILE | CYS | GLY | GLU | GLY | ||||
| 5 | THR | PRO | GLN | VAL | THR | GLY | PHE | PHE | GLU | VAL | ||||
| 6 | THR | VAL | ALA | GLY | LYS | LEU | VAL | HIS | SER | LYS | ||||
| 7 | LYS | ARG | GLY | ASP | GLY | TYR | VAL | ASP | THR | GLU | ||||
| 8 | SER | LYS | PHE | ARG | LYS | LEU | VAL | THR | ALA | ILE | ||||
| 9 | LYS | ALA | ALA | LEU | ALA | GLN | CYS | GLN | LEU | GLU | ||||
| 10 | HIS | HIS | HIS | HIS | HIS | HIS |
Samples:
sample_1: SelW, [U-98% 13C; U-99% 15N], 1 ± 0.1 mM; phosphate buffer 25 mM; NaCl 50 mM; DTT 2.5 mM; H2O 90%; D2O 10%
conditions_1: pH: 5.1; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H15N_HSQC | sample_1 | not available | conditions_1 |
| 1H13C_HSQC | sample_1 | not available | conditions_1 |
| 2D_1H_NOESY | sample_1 | not available | conditions_1 |
| 2D_1H_TOCSY | sample_1 | not available | conditions_1 |
| 2D_1H_IP-COSY | sample_1 | not available | conditions_1 |
| 3D_15N-separated_NOESY | sample_1 | not available | conditions_1 |
| 3D_13C-separated_NOESY | sample_1 | not available | conditions_1 |
| HNCA | sample_1 | not available | conditions_1 |
| HN(CO)CA | sample_1 | not available | conditions_1 |
| HN(CA)CO | sample_1 | not available | conditions_1 |
| HNCO | sample_1 | not available | conditions_1 |
| CACBNH | sample_1 | not available | conditions_1 |
| CACB(CO)NH | sample_1 | not available | conditions_1 |
| HAHBNH | sample_1 | not available | conditions_1 |
| HAHB(CO)NH | sample_1 | not available | conditions_1 |
| HCCH-TOCSY | sample_1 | not available | conditions_1 |
| HCCH-COSY | sample_1 | not available | conditions_1 |
Software:
CARA v1.4 - data analysis
xwinnmr v3.5 - collection
NMR spectrometers:
- Bruker Avence AV 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAC55247 BAC55256 |
| GB | AAB69860 AAC52255 AAC53317 AAF64481 AAH52719 |
| REF | NP_033182 NP_037159 XP_005139276 XP_005372482 |
| SP | P63300 P63301 |
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