BMRB Entry 7354
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PDB ID: 2bjc
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7354
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Title: NMR STRUCTURE OF A PROTEIN-DNA COMPLEX OF AN ALTERED SPECIFICITY MUTANT OF THE LAC REPRESSOR THAT MIMICS THE GAL REPRESSOR PubMed: 16094693
Deposition date: 2006-12-01 Original release date: 2010-02-25
Authors: Salinas, R.; Folkers, G.; Bonvin, A.; Das, D.; Boelens, R.; Kaptein, R.
Citation: Kopke Salinas, Roberto; Folkers, Gert; Bonvin, Alexandre; Das, Devashish; Boelens, Rolf; Kaptein, Robert. "Altered specificity in DNA binding by the lac repressor: a mutant lac headpiece that mimics the gal repressor" Chembiochem 6, 1628-1637 (2005).
Assembly members:
LAC-GAL_OPERATOR, polymer, 22 residues, Formula weight is not available
LAC_HEADPIECE_1-62, polymer, 62 residues, Formula weight is not available
Natural source: Common Name: ESCHERICHIA COLI Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: ESCHERICHIA COLI
Entity Sequences (FASTA):
LAC-GAL_OPERATOR: GAATTGTAAGCGCTTACAAT
TC
LAC_HEADPIECE_1-62: MKPVTLYDVAEYAGVSVATV
SRVVNQASHVSAKTREKVEA
AMAELNYIPNRCAQQLAGKQ
SL
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 248 |
| 15N chemical shifts | 70 |
| 1H chemical shifts | 544 |
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