BMRB Entry 17966
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17966
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Title: PDGFR beta-TM PubMed: 22619173
Deposition date: 2011-09-30 Original release date: 2012-09-20
Authors: Muhle-Goll, Claudia; Hoffmann, Silke; Ulrich, Anne
Citation: Muhle-Goll, Claudia; Hoffmann, Silke; Afonin, Sergii; Grage, Stephan; Polyansky, Anton; Windisch, Dirk; Zeitler, Marcel; Burck, Jochen; Ulrich, Anne. "Hydrophobic Matching Controls the Tilt and Stability of the Dimeric Platelet-derived Growth Factor Receptor (PDGFR) Transmembrane Segment." J. Biol. Chem. 287, 26178-26186 (2012).
Assembly members:
entity, polymer, 39 residues, 4421.575 Da.
Natural source: Common Name: cow Taxonomy ID: 9913 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Bos taurus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity: GHSLPFKVVVISAILALVVL
TIISLIILIMLWQKKPRYE
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 153 |
15N chemical shifts | 33 |
1H chemical shifts | 289 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | PDGFR-TM, chain 1 | 1 |
2 | PDGFR-TM, chain 2 | 1 |
Entities:
Entity 1, PDGFR-TM, chain 1 39 residues - 4421.575 Da.
1 | GLY | HIS | SER | LEU | PRO | PHE | LYS | VAL | VAL | VAL | ||||
2 | ILE | SER | ALA | ILE | LEU | ALA | LEU | VAL | VAL | LEU | ||||
3 | THR | ILE | ILE | SER | LEU | ILE | ILE | LEU | ILE | MET | ||||
4 | LEU | TRP | GLN | LYS | LYS | PRO | ARG | TYR | GLU |
Samples:
sample_1: PDGFR-TM, [U-100% 15N], 1 mM; DPC, [U-2H], 200 mM
sample_2: PDGFR-TM, [U-100% 13C; U-100% 15N], 1 mM; DPC, [U-2H], 200 mM
sample_3: PDGFR-TM, [U-100% 13C; U-100% 15N], 1 mM; PDGFR-TM 1 mM; DPC 200 mM
sample_4: PDGFR-TM, [U-100% 13C; U-100% 15N], 1 mM; DPC, [U-2H], 200 mM
sample_conditions_1: ionic strength: 120 mM; pH: 6.8; pressure: 1 atm; temperature: 323 K
sample_conditions_2: ionic strength: 120 mM; pH: 6.8; pressure: 1 atm; temperature: 310 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_2 |
3D HNCACB | sample_2 | isotropic | sample_conditions_1 |
3D HNCA | sample_2 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_2 | isotropic | sample_conditions_1 |
3D C(CO)NH | sample_2 | isotropic | sample_conditions_1 |
3D 1H-13C NOESY | sample_3 | isotropic | sample_conditions_1 |
3D 1H-13C NOESY | sample_3 | isotropic | sample_conditions_2 |
3D 13C-filtered 13C-edited NOESY | sample_4 | isotropic | sample_conditions_2 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRView, Johnson, One Moon Scientific - data analysis
ARIA v1.2, Linge, O, . - structure solution
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 900 MHz
Related Database Links:
PDB | |
DBJ | BAD92894 BAE22283 BAE34383 BAF85782 BAG11039 |
EMBL | CAA27882 |
GB | AAA36427 AAA60049 AAH32224 AAH55311 AAS48371 |
PRF | 1210315A |
REF | NP_001003382 NP_001069364 NP_001139740 NP_002600 NP_032835 |
SP | P05622 P09619 Q6QNF3 |
TPG | DAA27302 |
Download simulated HSQC data in one of the following formats:
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SPARKY: Backbone
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