BMRB Entry 18248
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18248
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Title: Assignments for the dually targeting peptide from Thr-tRNA synthetase PubMed: 22863398
Deposition date: 2012-02-09 Original release date: 2012-08-27
Authors: Ye, Weihua; Spanning, Erika; Unnerstale, Sofia; Gotthold, David; Glaser, Elzbieta; Maler, Lena
Citation: Ye, Weihua; Spanning, Erika; Unnerstale, Sofia; Gotthold, David; Glaser, Elzbieta; Maler, Lena. "NMR investigations of the dual targeting peptide of Thr-tRNA synthetase and its interaction with the mitochondrial Tom20 receptor in Arabidopsisthaliana." FEBS J. 279, 3738-3748 (2012).
Assembly members:
ThrRS-dTP(2-60), polymer, 59 residues, Formula weight is not available
Natural source: Common Name: Thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
ThrRS-dTP(2-60): ASSHSLLFSSSFLSKPSSFT
SSLRRFVYLPTRQFWPRQRH
GFSTVFAVATEPAISSSGP
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 49 |
15N chemical shifts | 51 |
1H chemical shifts | 277 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | ThrRS-dTP | 1 |
Entities:
Entity 1, ThrRS-dTP 59 residues - Formula weight is not available
1 | ALA | SER | SER | HIS | SER | LEU | LEU | PHE | SER | SER | ||||
2 | SER | PHE | LEU | SER | LYS | PRO | SER | SER | PHE | THR | ||||
3 | SER | SER | LEU | ARG | ARG | PHE | VAL | TYR | LEU | PRO | ||||
4 | THR | ARG | GLN | PHE | TRP | PRO | ARG | GLN | ARG | HIS | ||||
5 | GLY | PHE | SER | THR | VAL | PHE | ALA | VAL | ALA | THR | ||||
6 | GLU | PRO | ALA | ILE | SER | SER | SER | GLY | PRO |
Samples:
sample_1: ThrRS-dTP(2-60), [U-15N], 80 100 uM; D2O, [U-100% 2H], 10%; sodium phosphate 50 mM
sample_2: ThrRS-dTP(2-60), [U-13C; U-15N], 80 100 uM; D2O, [U-100% 2H], 10%; sodium phosphate 50 mM
sample_conditions_1: pH: 6.0; temperature: 298 K
sample_conditions_2: pH: 6.0; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
HNCA | sample_2 | isotropic | sample_conditions_2 |
HN(CO)CA | sample_2 | isotropic | sample_conditions_2 |
Software:
SPARKY, Goddard - chemical shift assignment
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 700 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts