BMRB Entry 18815

Name: N-histidine-tagged EIAV-CA
Published: 2012-11-07

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18815

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: N-histidine-tagged EIAV-CA

Deposition date: 2012-10-31 Original release date: 2012-11-07

Authors: Chen, Kang; Tjandra, Nico

Citation: Chen, Kang. "eiav-ca" Not known ., .-..

Assembly members:
his-eiav-ca, polymer, 237 residues, Formula weight is not available

Natural source: Common Name: swamp fever Taxonomy ID: 11665 Superkingdom: Viruses Kingdom: not available Genus/species: Lentivirus not available

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
his-eiav-ca: AHHHHHHGPIMIDGAGNRNF RPLTPRGYTTWVNTIQTNGL LNEASQNLFGILSVDCTSEE MNAFLDVVPGQAGQKQILLD AIDKIADDWDNRHPLPNAPL VAPPQGPIPMTARFIRGLGV PRERQMEPAFDQFRQTYRQW IIEAMSEGIKVMIGKPKAQN IRQGAKEPYPEFVDRLLSQI KSEGHPQEISKFLTDTLTIQ NANEECRNAMRHLRPEDTLE EKMYACRDIGTTKQKMM

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	650
15N chemical shifts	207
1H chemical shifts	207

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	his-eiav-ca	1

Entities:

Entity 1, his-eiav-ca 237 residues - Formula weight is not available

1

ALA

HIS

GLY

PRO

ILE

2

MET

ILE

ASP

GLY

ALA

GLY

ASN

ARG

ASN

PHE

3

ARG

PRO

LEU

THR

PRO

ARG

GLY

TYR

THR

4

TRP

VAL

ASN

THR

ILE

GLN

THR

ASN

GLY

LEU

5

LEU

ASN

GLU

ALA

SER

GLN

ASN

LEU

PHE

GLY

6

ILE

LEU

SER

VAL

ASP

CYS

THR

SER

GLU

7

MET

ASN

ALA

PHE

LEU

ASP

VAL

PRO

GLY

8

GLN

ALA

GLY

GLN

LYS

GLN

ILE

LEU

ASP

9

ALA

ILE

ASP

LYS

ILE

ALA

ASP

TRP

ASP

10

ASN

ARG

HIS

PRO

LEU

PRO

ASN

ALA

PRO

LEU

11

VAL

ALA

PRO

GLN

GLY

PRO

ILE

PRO

MET

12

THR

ALA

ARG

PHE

ILE

ARG

GLY

LEU

GLY

VAL

13

PRO

ARG

GLU

ARG

GLN

MET

GLU

PRO

ALA

PHE

14

ASP

GLN

PHE

ARG

GLN

THR

TYR

ARG

GLN

TRP

15

ILE

GLU

ALA

MET

SER

GLU

GLY

ILE

LYS

16

VAL

MET

ILE

GLY

LYS

PRO

LYS

ALA

GLN

ASN

17

ILE

ARG

GLN

GLY

ALA

LYS

GLU

PRO

TYR

PRO

18

GLU

PHE

VAL

ASP

ARG

LEU

SER

GLN

ILE

19

LYS

SER

GLU

GLY

HIS

PRO

GLN

GLU

ILE

SER

20

LYS

PHE

LEU

THR

ASP

THR

LEU

THR

ILE

GLN

21

ASN

ALA

ASN

GLU

CYS

ARG

ASN

ALA

MET

22

ARG

HIS

LEU

ARG

PRO

GLU

ASP

THR

LEU

GLU

23

GLU

LYS

MET

TYR

ALA

CYS

ARG

ASP

ILE

GLY

24

THR

LYS

GLN

LYS

MET

Samples:

sample_1: his-eiav-ca, [U-13C; U-15N; U-2H], 0.15 mM; H2O 93%; D2O 7%

sample_conditions_1: ionic strength: 0 M; pH: 6.7; pressure: 1 atm; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(COCA)CB	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz
Bruker Avance 900 MHz

BMRB	18421
PDB	1EIA 2EIA
DBJ	BAB12103 BAB12109
GB	AAA43003 AAA43010 AAA43011 AAB59861 AAC03760
REF	NP_056901
SP	P69730 P69731 P69732

Biological Magnetic Resonance Data Bank