BMRB Entry 25179
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25179
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Title: Backbone resonance assignments of the wt NS4A N-terminal domain of DENV PubMed: 25644870
Deposition date: 2014-08-29 Original release date: 2015-03-25
Authors: Willbold, Dieter; Koenig, Bernd; Hung, Yu-Fu; Schwarten, Melanie
Citation: Hung, Yu-Fu; Schuenke, Sven; Schwarten, Melanie; Thiagarajan, Pallavi; Hoffmann, Silke; Sklan, Ella; Willbold, Dieter; Koenig, Bernd. "Dengue virus NS4A cytoplasmic domain binding to liposomes is sensitive to membrane curvature" Biochim. Biophys. Acta 1848, 1119-1126 (2015).
Assembly members:
wtNS4A, polymer, 48 residues, 5226.9633 Da.
Natural source: Common Name: Dengue virus 2 Taxonomy ID: 11060 Superkingdom: Viruses Kingdom: not available Genus/species: Flavivirus Dengue virus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
wtNS4A: SLTLNLITEMGRLPTFMTQK
ARDALDNLAVLHTAEAGGRA
YNHALSEL
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 168 |
| 13C chemical shifts | 137 |
| 15N chemical shifts | 46 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | wtNS4A | 1 |
Entities:
Entity 1, wtNS4A 48 residues - 5226.9633 Da.
| 1 | SER | LEU | THR | LEU | ASN | LEU | ILE | THR | GLU | MET | ||||
| 2 | GLY | ARG | LEU | PRO | THR | PHE | MET | THR | GLN | LYS | ||||
| 3 | ALA | ARG | ASP | ALA | LEU | ASP | ASN | LEU | ALA | VAL | ||||
| 4 | LEU | HIS | THR | ALA | GLU | ALA | GLY | GLY | ARG | ALA | ||||
| 5 | TYR | ASN | HIS | ALA | LEU | SER | GLU | LEU |
Samples:
NMR_sample: wtNS4A, [U-13C; U-15N], 1.0 mM; Sodium Dodecyl Sulfate, [U-2H], 100.0 mM; Sodium Phosphate 50.0 mM; sodium azide 0.03 % w/v; H2O 90%; D2O 10%
NMR_sample_ref: wtNS4A, [U-13C; U-15N], 1.0 mM; Sodium Dodecyl Sulfate, [U-2H], 100.0 mM; Sodium Phosphate 50.0 mM; DSS 10 mM; sodium azide 0.03 % w/v; H2O 90%; D2O 10%
Set_1: ionic strength: 150.000 mM; pH: 6.800; pressure: 1.000 atm; temperature: 303.000 K
Set_2: ionic strength: 150.000 mM; pH: 6.800; pressure: 1.000 atm; temperature: 303.000 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC/HMQC | NMR_sample | isotropic | Set_1 |
| 3D HNCACB | NMR_sample | isotropic | Set_1 |
| HNHA (H[N[ca[HA]]]) | NMR_sample | isotropic | Set_1 |
| 3D HNCO | NMR_sample | isotropic | Set_1 |
| 2D 1H-13C HSQC/HMQC | NMR_sample_ref | isotropic | Set_2 |
| 2D 1H-13C HSQC/HMQC | NMR_sample | isotropic | Set_1 |
| 2D 1H-15N HSQC/HMQC | NMR_sample | isotropic | Set_1 |
Software:
CcpNmr_Analysis v2.4, CCPN - chemical shift assignment
NMRPipe v8.1, NIH - processing
TALOS-N v4.01, Yang Shen and Ad Bax - data analysis
NMR spectrometers:
- Varian VNMRS 900 MHz
- Varian VNMRS 800 MHz
Related Database Links:
| GenBank | np739588 |
| BMRB | 25586 |
| DBJ | BAD42415 BAD42416 BAD42417 BAH86603 BAH86604 |
| EMBL | CAD31751 CAI92123 CAR65133 CAR65134 CAR65135 |
| GB | AAA42941 AAA42942 AAA42962 AAA73185 AAA73186 |
| REF | NP_056776 NP_739588 |
| SP | P07564 P12823 P14337 P14340 P29990 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts