BMRB Entry 25354
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25354
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Title: SH3 domain from yeast Abp1p PubMed: 25569628
Deposition date: 2014-11-20 Original release date: 2014-12-19
Authors: Lundstrom, Patrik
Citation: Niklasson, Markus; Ahlner, Alexandra; Andresen, Cecilia; Marsh, Joseph; Lundstrom, Patrik. "Fast and accurate resonance assignment of small-to-large proteins by combining automated and manual approaches" PLOS Comput. Biol. 11, e1004022-e1004022 (2015).
Assembly members:
Abp1p_SH3, polymer, 62 residues, Formula weight is not available
Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Abp1p_SH3: GAMAPWATAEYDYDAAEDNE
LTFVENDKIINIEFVDDDWW
LGELEKDGSKGLFPSNYVSL
GN
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 215 |
| 15N chemical shifts | 57 |
| 1H chemical shifts | 311 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Abp1p SH3 domain | 1 |
Entities:
Entity 1, Abp1p SH3 domain 62 residues - Formula weight is not available
| 1 | GLY | ALA | MET | ALA | PRO | TRP | ALA | THR | ALA | GLU | ||||
| 2 | TYR | ASP | TYR | ASP | ALA | ALA | GLU | ASP | ASN | GLU | ||||
| 3 | LEU | THR | PHE | VAL | GLU | ASN | ASP | LYS | ILE | ILE | ||||
| 4 | ASN | ILE | GLU | PHE | VAL | ASP | ASP | ASP | TRP | TRP | ||||
| 5 | LEU | GLY | GLU | LEU | GLU | LYS | ASP | GLY | SER | LYS | ||||
| 6 | GLY | LEU | PHE | PRO | SER | ASN | TYR | VAL | SER | LEU | ||||
| 7 | GLY | ASN |
Samples:
sample_1: Abp1p SH3, [U-13C; U-15N], 0.5 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 150 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
Software:
COMPASS, Niklasson, Ahlner, Andresen, Marsh, Lundstrom - chemical shift assignment
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| BMRB | 16843 18054 18055 18056 18057 18058 18059 18060 18061 18062 18063 18064 18065 18066 18067 18068 18069 18070 18071 18072 18073 18074 18075 18076 18077 18078 26519 |
| PDB | |
| DBJ | GAA21988 |
| EMBL | CAA36075 CAA42253 CAY78291 |
| GB | AHN96147 AHV79349 AHY79755 AJP37506 AJQ31991 |
| PRF | 1603360A |
| REF | NP_010012 |
| SP | P15891 |
| TPG | DAA07557 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts