BMRB Entry 25410
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR25410
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Backbone assignments of a construct of the Sigma-1 receptor spanning residues 35-223 PubMed: 25647032
Deposition date: 2015-01-04 Original release date: 2016-06-30
Authors: Ortega-Roldan, Jose L.; Schnell, Jason
Citation: Ortega-Roldan, Jose Luis; Ossa, Felipe; Amin, Nader; Schnell, Jason. "Solution NMR studies reveal the location of the second transmembrane domain of the human sigma-1 receptor" FEBS Lett. 589, 659-665 (2015).
Assembly members:
Sigma-1_Receptor_delta35, polymer, 275 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Sigma-1_Receptor_delta35: MNHKVHHHHHHIEGRHMFVF
QREEIAQLARQYAGLDHELA
FSRLIVELRRLHPGHVLPDE
ELQWVFVNAGGWMGAMALLH
ASLSEYVLLFGTALGSRGHS
GRYWAEISDTIISGTFHQWR
EGTTKSEVFYPGETVVHGPG
EATAVEWGPNTWMVEYGRGV
IPSTLAFALADTVFSTQDFL
TLFYTLRSYARGLRLELTTY
LFGQDPGPGEATAVEWGPNT
WMVEYGRGVIPSTLAFALAD
TVFSTQDFLTLFYTLRSYAR
GLRLELTTYLFGQDP
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 429 |
| 15N chemical shifts | 169 |
| 1H chemical shifts | 168 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Sigma-1 receptor delta 35 construct | 1 |
Entities:
Entity 1, Sigma-1 receptor delta 35 construct 275 residues - Formula weight is not available
| 1 | MET | ASN | HIS | LYS | VAL | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | HIS | ILE | GLU | GLY | ARG | HIS | MET | PHE | VAL | PHE | ||||
| 3 | GLN | ARG | GLU | GLU | ILE | ALA | GLN | LEU | ALA | ARG | ||||
| 4 | GLN | TYR | ALA | GLY | LEU | ASP | HIS | GLU | LEU | ALA | ||||
| 5 | PHE | SER | ARG | LEU | ILE | VAL | GLU | LEU | ARG | ARG | ||||
| 6 | LEU | HIS | PRO | GLY | HIS | VAL | LEU | PRO | ASP | GLU | ||||
| 7 | GLU | LEU | GLN | TRP | VAL | PHE | VAL | ASN | ALA | GLY | ||||
| 8 | GLY | TRP | MET | GLY | ALA | MET | ALA | LEU | LEU | HIS | ||||
| 9 | ALA | SER | LEU | SER | GLU | TYR | VAL | LEU | LEU | PHE | ||||
| 10 | GLY | THR | ALA | LEU | GLY | SER | ARG | GLY | HIS | SER | ||||
| 11 | GLY | ARG | TYR | TRP | ALA | GLU | ILE | SER | ASP | THR | ||||
| 12 | ILE | ILE | SER | GLY | THR | PHE | HIS | GLN | TRP | ARG | ||||
| 13 | GLU | GLY | THR | THR | LYS | SER | GLU | VAL | PHE | TYR | ||||
| 14 | PRO | GLY | GLU | THR | VAL | VAL | HIS | GLY | PRO | GLY | ||||
| 15 | GLU | ALA | THR | ALA | VAL | GLU | TRP | GLY | PRO | ASN | ||||
| 16 | THR | TRP | MET | VAL | GLU | TYR | GLY | ARG | GLY | VAL | ||||
| 17 | ILE | PRO | SER | THR | LEU | ALA | PHE | ALA | LEU | ALA | ||||
| 18 | ASP | THR | VAL | PHE | SER | THR | GLN | ASP | PHE | LEU | ||||
| 19 | THR | LEU | PHE | TYR | THR | LEU | ARG | SER | TYR | ALA | ||||
| 20 | ARG | GLY | LEU | ARG | LEU | GLU | LEU | THR | THR | TYR | ||||
| 21 | LEU | PHE | GLY | GLN | ASP | PRO | GLY | PRO | GLY | GLU | ||||
| 22 | ALA | THR | ALA | VAL | GLU | TRP | GLY | PRO | ASN | THR | ||||
| 23 | TRP | MET | VAL | GLU | TYR | GLY | ARG | GLY | VAL | ILE | ||||
| 24 | PRO | SER | THR | LEU | ALA | PHE | ALA | LEU | ALA | ASP | ||||
| 25 | THR | VAL | PHE | SER | THR | GLN | ASP | PHE | LEU | THR | ||||
| 26 | LEU | PHE | TYR | THR | LEU | ARG | SER | TYR | ALA | ARG | ||||
| 27 | GLY | LEU | ARG | LEU | GLU | LEU | THR | THR | TYR | LEU | ||||
| 28 | PHE | GLY | GLN | ASP | PRO |
Samples:
S1chtriple: Sigma-1 Receptor delta35, [U-100% 13C; U-100% 15N; U-80% 2H], 0.27 mM; H2O 95%; D2O 5%
S1chdoble: Sigma-1 Receptor delta35, [U-100% 13C; U-100% 15N], 0.27 mM; H2O 95%; D2O 5%
sample_conditions_1: ionic strength: 0 M; pH: 6.5; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | S1chtriple | isotropic | sample_conditions_1 |
| 3D HNCA | S1chtriple | isotropic | sample_conditions_1 |
| 3D HNCACB | S1chtriple | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | S1chdoble | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | S1chdoble | isotropic | sample_conditions_1 |
| 3D HNCO | S1chtriple | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | S1chtriple | isotropic | sample_conditions_1 |
Software:
NMRView, Johnson, One Moon Scientific - chemical shift assignment
NMR spectrometers:
- Bruker DRX 600 MHz
- Bruker DRX 500 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts