BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 25627

Title: Solution structure of the meiosis-expressed gene 1 (Meig1)   PubMed: 26726850

Deposition date: 2015-05-16 Original release date: 2016-01-19

Authors: Williams, David; Walavalkar, Ninad; Buchwald, William

Citation: Li, Wei; Walavalkar, Ninad; Buchwald, William; Teves, Maria; Zhang, Ling; Liu, Junping; Bilinovich, S.; Peterson, Darrell; Strauss, Jerome; Williams, David; Zhang, Zhibing. "Dissecting the structural basis of MEIG1 interaction with PACRG"  Sci. Rep. 6, 18278-18278 (2016).

Assembly members:
entity, polymer, 92 residues, 11256.876 Da.

Natural source:   Common Name: house mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity: GSHMMATSDVKPKSISRAKK WSEEIENLYRFQQAGYRDEI EYKQVKQVAMVDRWPETGYV KKLQRRDNTFFYYNKERECE DKEVHKVKVYVY

Data sets:
Data typeCount
13C chemical shifts410
15N chemical shifts99
1H chemical shifts623

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 92 residues - 11256.876 Da.

1   GLYSERHISMETMETALATHRSERASPVAL
2   LYSPROLYSSERILESERARGALALYSLYS
3   TRPSERGLUGLUILEGLUASNLEUTYRARG
4   PHEGLNGLNALAGLYTYRARGASPGLUILE
5   GLUTYRLYSGLNVALLYSGLNVALALAMET
6   VALASPARGTRPPROGLUTHRGLYTYRVAL
7   LYSLYSLEUGLNARGARGASPASNTHRPHE
8   PHETYRTYRASNLYSGLUARGGLUCYSGLU
9   ASPLYSGLUVALHISLYSVALLYSVALTYR
10   VALTYR

Samples:

sample_1: sodium phosphate 10 mM; Meig1, [U-99% 13C; U-99% 15N], 0.5 – 1 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 40 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
2D IPAP-HSQCsample_1anisotropicsample_conditions_1
2D IPAP-HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CcpNMR, CCPN - data analysis

X-PLOR_NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Bruker Avance 850 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts