BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 25745

Title: Structure of the N-terminal domain of the metalloprotease PrtV from Vibrio cholerae   PubMed: 26434928

Deposition date: 2015-08-07 Original release date: 2015-08-24

Authors: Persson, Cecilia; Mayzel, M.; Edwin, A.; Wai, S.; Ohman, A.; Sauer-eriksson, A.; Karlsson, G.

Citation: Edwin, A.; Mayzel, M.; Persson, Cecilia; Wai, S.; Ohman, A.; Karlsson, G.; Sauer-eriksson, A.. "Structure of the N-terminal domain of the metalloprotease PrtV from Vibrio cholerae"  Protein Sci. 24, 2076-2080 (2015).

Assembly members:
METALLOPROTEASE, polymer, 84 residues, 9348.8769 Da.

Natural source:   Common Name: Vibrio cholerae   Taxonomy ID: 666   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Vibrio cholerae

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
METALLOPROTEASE: GAMAQTPIDLGVVNEDKLIE MLVRTGQIPADASDVDKRIA LERYLEEKIRSGFKGDAQFG KKALEQRAKILKVIDKQKGP HKAR

Data sets:
Data typeCount
13C chemical shifts357
15N chemical shifts79
1H chemical shifts549

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1METALLOPROTEASE1

Entities:

Entity 1, METALLOPROTEASE 84 residues - 9348.8769 Da.

1   GLYALAMETALAGLNTHRPROILEASPLEU
2   GLYVALVALASNGLUASPLYSLEUILEGLU
3   METLEUVALARGTHRGLYGLNILEPROALA
4   ASPALASERASPVALASPLYSARGILEALA
5   LEUGLUARGTYRLEUGLUGLULYSILEARG
6   SERGLYPHELYSGLYASPALAGLNPHEGLY
7   LYSLYSALALEUGLUGLNARGALALYSILE
8   LEULYSVALILEASPLYSGLNLYSGLYPRO
9   HISLYSALAARG

Samples:

sample_1: METALLOPROTEASE, [U-13C; U-15N], 0.7 mM; Pi 20 mM

sample_conditions_1: pH: 7.000; pressure: 1 atm; temperature: 283.000 K

Experiments:

NameSampleSample stateSample conditions
Backbone expssample_1solutionsample_conditions_1
NOE expssample_1solutionsample_conditions_1

Software:

AutoDep v4.3, PDBe - chemical shift assignment

ccpn vany, CCPN - chemical shift assignment

x-plor vany, Brunger - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts