BMRB Entry 25875
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25875
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Title: Backbone assignment of Calcium loaded C-terminus of Troponin C isoform 2 PubMed: 27226601
Deposition date: 2015-11-02 Original release date: 2016-01-29
Authors: Sanfelice, Domenico; Pastore, Annalisa; Adrover, Miguel
Citation: Sanfelice, Domenico; Pastore, Annalisa; Bullard, Belinda. "Towards Understanding the Molecular Bases of Stretch Activation: A Structural comparison of the Two Troponin C Isoforms of Lethocerus" J. Biol. Chem. 291, 16090-16099 (2016).
Assembly members:
Cterm_TnC_F2, polymer, 71 residues, Formula weight is not available
Natural source: Common Name: Lethocerus Indicus Taxonomy ID: 212017 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Lethocerus Indicus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Cterm_TnC_F2: GMMEELREAFRLYDKDGNGY
ITTEVLREILKELDDKITPE
DLDMMIQEIDSDGSGTVDFD
EFMEVMIGGDE
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 205 |
15N chemical shifts | 66 |
1H chemical shifts | 356 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | C-terminus F2 TnC | 1 |
Entities:
Entity 1, C-terminus F2 TnC 71 residues - Formula weight is not available
seq starts at 88
1 | GLY | MET | MET | GLU | GLU | LEU | ARG | GLU | ALA | PHE | ||||
2 | ARG | LEU | TYR | ASP | LYS | ASP | GLY | ASN | GLY | TYR | ||||
3 | ILE | THR | THR | GLU | VAL | LEU | ARG | GLU | ILE | LEU | ||||
4 | LYS | GLU | LEU | ASP | ASP | LYS | ILE | THR | PRO | GLU | ||||
5 | ASP | LEU | ASP | MET | MET | ILE | GLN | GLU | ILE | ASP | ||||
6 | SER | ASP | GLY | SER | GLY | THR | VAL | ASP | PHE | ASP | ||||
7 | GLU | PHE | MET | GLU | VAL | MET | ILE | GLY | GLY | ASP | ||||
8 | GLU |
Samples:
sample_1: Cterm TnC F2, [U-98% 13C; U-98% 15N], 0.5 1.0 mM; D2O, [U-2H], 10%; H2O 90%
sample_conditions_1: ionic strength: 0.1 M; pH: 6.8; pressure: 1 atm; temperature: 298.2 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
XEASY v1.2, Bartels et al. - chemical shift assignment
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 700 MHz
- Bruker Avance 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts