BMRB Entry 25935
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25935
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Title: Membrane-bound mouse CD28 cytoplasmic tail
Deposition date: 2015-12-23 Original release date: 2016-12-15
Authors: Li, Hua; Xu, Chenqi; Pan, Weiling
Citation: Li, Hua; Xu, Chenqi; Pan, Weiling. "Membrane-bound mouse CD28 cytoplasmic tail" . ., .-..
Assembly members:
entity, polymer, 43 residues, 5093.833 Da.
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity: GTNSRRNRLLQSDYMNMTPR
RPGLTRKPYQPYAPARDFAA
YRP
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 188 |
| 15N chemical shifts | 48 |
| 1H chemical shifts | 308 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity | 1 |
Entities:
Entity 1, entity 43 residues - 5093.833 Da.
| 1 | GLY | THR | ASN | SER | ARG | ARG | ASN | ARG | LEU | LEU | ||||
| 2 | GLN | SER | ASP | TYR | MET | ASN | MET | THR | PRO | ARG | ||||
| 3 | ARG | PRO | GLY | LEU | THR | ARG | LYS | PRO | TYR | GLN | ||||
| 4 | PRO | TYR | ALA | PRO | ALA | ARG | ASP | PHE | ALA | ALA | ||||
| 5 | TYR | ARG | PRO |
Samples:
sample_1: mCD28CD, [U-13C; U-15N], 0.5 mM; POPG 100 mM; DHPC 125 mM; sodium phosphate 20 mM; D2O, [U-2H], 10%; H2O 90%
sample_conditions_1: ionic strength: 20 mM; pH: 6.7; pressure: 1 atm; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY (NOEs to lipid) | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY (methyl; double 13C-filter) | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C NOESY (aromatic; double 13C-filter) | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v3.2, Bruker Biospin - collection
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRView v5.2.2, Johnson, One Moon Scientific - data analysis
KUJIRA v0.9843, Naohiro Kobayashi - data analysis
X-PLOR_NIH v2.32, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution
NMR spectrometers:
- Bruker Avance 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts