BMRB Entry 26650
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26650
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: SIX3_HD
Deposition date: 2015-09-07 Original release date: 2015-11-04
Authors: Sharma, Shrikant
Citation: AMPAPATHI, RAVI; Sharma, Shrikant. "Structural Studies of SIX3-HD and Rhodospin" Not known ., .-..
Assembly members:
SIX3_HD, polymer, 60 residues, Formula weight is not available
Natural source: Common Name: enterobacteria Taxonomy ID: 469008 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
SIX3_HD: DGEQKTHCFKERTRSLLREW
YLQDPYPNPSKKRELAQATG
LTPTQVGNWFKNRRQRDRAA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 260 |
| 15N chemical shifts | 62 |
| 1H chemical shifts | 419 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SIX3_HD | 1 |
Entities:
Entity 1, SIX3_HD 60 residues - Formula weight is not available
| 1 | ASP | GLY | GLU | GLN | LYS | THR | HIS | CYS | PHE | LYS | |
| 2 | GLU | ARG | THR | ARG | SER | LEU | LEU | ARG | GLU | TRP | |
| 3 | TYR | LEU | GLN | ASP | PRO | TYR | PRO | ASN | PRO | SER | |
| 4 | LYS | LYS | ARG | GLU | LEU | ALA | GLN | ALA | THR | GLY | |
| 5 | LEU | THR | PRO | THR | GLN | VAL | GLY | ASN | TRP | PHE | |
| 6 | LYS | ASN | ARG | ARG | GLN | ARG | ASP | ARG | ALA | ALA |
Samples:
sample_1: SIX3_HD, [U-100% 13C; U-100% 15N], 0.9 mM; sodium phosphate 50 mM; sodium chloride 150 mM; beta-mercaptoethanol 2 mM; sodium azide 1 mM; H2O 95%; D2O 5%
sample_conditions_1: ionic strength: 0.2 M; pH: 7.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aliphatic | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aromatic | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
SPARKY, Goddard - chemical shift assignment, data analysis
NMR spectrometers:
- Agilent DD2 700 MHz
Related Database Links:
| DBJ | BAA11822 BAA11823 BAA31752 BAA94484 BAA94485 |
| EMBL | CAA09773 CAA09774 CAA09775 CAA62379 CAA75380 |
| GB | AAC27448 AAC27449 AAC33850 AAC33851 AAD11939 |
| PIR | S74256 |
| REF | NP_001018421 NP_001079171 NP_001081933 NP_001093696 NP_001101502 |
| SP | O42406 O93307 O95343 O95475 Q62233 |
| TPG | DAA24767 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts