BMRB Entry 26735
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26735
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Title: LdcI Assocating Domain of RavA Backbone Assignment PubMed: 27265850
Deposition date: 2016-01-23 Original release date: 2016-06-08
Authors: Sharpe, Simon; Houry, Walid A.; Chan, Sze Wah Samuel
Citation: Chan, Sze Wah Samuel. "Mechanism of Amyloidogenesis of a Bacterial AAA+ Chaperone" Structure 24, 1095-1109 (2016).
Assembly members:
LARA3, polymer, 115 residues, 12609.5014 Da.
Natural source: Common Name: Escherichia coli (strain K12) Taxonomy ID: 511693 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia not available
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
LARA3: GHMQQSDKTALTVIRLGGIF
SRRQQYQLPVNVTASTLTLL
LQKPLKLHDMEVVHISFERS
ALEQWLSKGGEIRGKLNGIG
FAQKLNLEVDSAQHLVVRDV
SLQGSTLALPGSSAE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 359 |
| 15N chemical shifts | 111 |
| 1H chemical shifts | 111 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | LARA3 | 1 |
Entities:
Entity 1, LARA3 115 residues - 12609.5014 Da.
| 1 | GLY | HIS | MET | GLN | GLN | SER | ASP | LYS | THR | ALA | ||||
| 2 | LEU | THR | VAL | ILE | ARG | LEU | GLY | GLY | ILE | PHE | ||||
| 3 | SER | ARG | ARG | GLN | GLN | TYR | GLN | LEU | PRO | VAL | ||||
| 4 | ASN | VAL | THR | ALA | SER | THR | LEU | THR | LEU | LEU | ||||
| 5 | LEU | GLN | LYS | PRO | LEU | LYS | LEU | HIS | ASP | MET | ||||
| 6 | GLU | VAL | VAL | HIS | ILE | SER | PHE | GLU | ARG | SER | ||||
| 7 | ALA | LEU | GLU | GLN | TRP | LEU | SER | LYS | GLY | GLY | ||||
| 8 | GLU | ILE | ARG | GLY | LYS | LEU | ASN | GLY | ILE | GLY | ||||
| 9 | PHE | ALA | GLN | LYS | LEU | ASN | LEU | GLU | VAL | ASP | ||||
| 10 | SER | ALA | GLN | HIS | LEU | VAL | VAL | ARG | ASP | VAL | ||||
| 11 | SER | LEU | GLN | GLY | SER | THR | LEU | ALA | LEU | PRO | ||||
| 12 | GLY | SER | SER | ALA | GLU |
Samples:
LARA: LARA3, [[U-95% 13C; U-95% 15N]], 0.45 mM; H2O 90%; D2O 10%; potassium chloride 50 mM
Assignment: ionic strength: 50.000 mM; pH: 4.000; pressure: 1.000 atm; temperature: 280.150 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| COCBCA (H[N[co[{CA|ca[C]}]]]) | LARA | isotropic | Assignment |
| CCCONH (C_ccoNH.relayed) | LARA | isotropic | Assignment |
| 3D HNCACB | LARA | isotropic | Assignment |
| CACO (H[N[ca[CO]]]) | LARA | isotropic | Assignment |
| 3D HNCA | LARA | isotropic | Assignment |
| 3D HN(CO)CA | LARA | isotropic | Assignment |
| 3D HNCO | LARA | isotropic | Assignment |
| 2D 1H-15N HSQC/HMQC | LARA | isotropic | Assignment |
Software:
CcpNmr_Analysis v2.3, CCPN - Aid
NMR spectrometers:
- Bruker Avance 700 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts