BMRB Entry 26748
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26748
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Title: NMR study of Met-1 Human Angiogenin - 1H, 13C, 15N backbone and side chain resonance assignment PubMed: 27624767
Deposition date: 2016-02-19 Original release date: 2016-09-29
Authors: Tsika, Aikaterini; Chatzileontiadou, Dimitra; Leonidas, Demetres; Spyroulias, Georgios
Citation: Tsika, Aikaterini; Chatzileontiadou, Dimitra; Leonidas, Dimitra; Spyroulias, Georgios. "NMR study of Met-1 human Angiogenin: (1)H, (13)C, (15)N backbone and side-chain resonance assignment" Biomol. NMR Assign. 10, 379-383 (2016).
Assembly members:
Met-1_Human_Ang, polymer, 124 residues, 14274.1 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Met-1_Human_Ang: MQDNSRYTHFLTQHYDAKPQ
GRDDRYCESIMRRRGLTSPC
KDINTFIHGNKRSIKAICEN
KNGNPHRENLRISKSSFQVT
TCKLHGGSPWPPCQYRATAG
FRNVVVACENGLPVHLDQSI
FRRP
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 469 |
15N chemical shifts | 112 |
1H chemical shifts | 714 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | Met-1 Human Ang | 1 |
Entities:
Entity 1, Met-1 Human Ang 124 residues - 14274.1 Da.
1 | MET | GLN | ASP | ASN | SER | ARG | TYR | THR | HIS | PHE | ||||
2 | LEU | THR | GLN | HIS | TYR | ASP | ALA | LYS | PRO | GLN | ||||
3 | GLY | ARG | ASP | ASP | ARG | TYR | CYS | GLU | SER | ILE | ||||
4 | MET | ARG | ARG | ARG | GLY | LEU | THR | SER | PRO | CYS | ||||
5 | LYS | ASP | ILE | ASN | THR | PHE | ILE | HIS | GLY | ASN | ||||
6 | LYS | ARG | SER | ILE | LYS | ALA | ILE | CYS | GLU | ASN | ||||
7 | LYS | ASN | GLY | ASN | PRO | HIS | ARG | GLU | ASN | LEU | ||||
8 | ARG | ILE | SER | LYS | SER | SER | PHE | GLN | VAL | THR | ||||
9 | THR | CYS | LYS | LEU | HIS | GLY | GLY | SER | PRO | TRP | ||||
10 | PRO | PRO | CYS | GLN | TYR | ARG | ALA | THR | ALA | GLY | ||||
11 | PHE | ARG | ASN | VAL | VAL | VAL | ALA | CYS | GLU | ASN | ||||
12 | GLY | LEU | PRO | VAL | HIS | LEU | ASP | GLN | SER | ILE | ||||
13 | PHE | ARG | ARG | PRO |
Samples:
sample_1: Met-1 Human Ang, [U-99% 15N], 0.6 mM; Sodium Acetate 50 mM; EDTA 0.2 mM
sample_2: Met-1 Human Ang, [U-99% 13C; U-99% 15N], 0.6 mM; Sodium Acetate 50 mM; EDTA 0.2 mM
sample_3: Met-1 Human Ang, [U-99% 15N], 0.3 mM; Sodium Acetate 50 mM; EDTA 0.2 mM
sample_conditions_1: pH: 5; pressure: 1 atm; temperature: 298 K
sample_conditions_2: pH: 5; pressure: 1 atm; temperature: 298 K
sample_conditions_3: pH: 5; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
2D 1H-15N HSQC | sample_3 | isotropic | sample_conditions_3 |
2D 1H-13C HSQC | sample_2 | isotropic | sample_conditions_2 |
3D CBCA(CO)NH | sample_2 | isotropic | sample_conditions_2 |
3D HNCACB | sample_2 | isotropic | sample_conditions_2 |
3D HNCA | sample_2 | isotropic | sample_conditions_2 |
3D HNCO | sample_2 | isotropic | sample_conditions_2 |
3D HN(CO)CA | sample_2 | isotropic | sample_conditions_2 |
3D HCCH-TOCSY | sample_2 | isotropic | sample_conditions_2 |
3D HBHA(CO)NH | sample_2 | isotropic | sample_conditions_2 |
2D 1H-13C HSQC | sample_2 | isotropic | sample_conditions_2 |
2D 1H-13C HSQC aliphatic | sample_2 | isotropic | sample_conditions_2 |
2D 1H-13C HSQC aromatic | sample_2 | isotropic | sample_conditions_2 |
3D HNHA | sample_1 | isotropic | sample_conditions_1 |
3D 1H-13C NOESY aliphatic | sample_2 | isotropic | sample_conditions_2 |
3D 1H-13C NOESY aromatic | sample_2 | isotropic | sample_conditions_2 |
3D H(CA)CO | sample_2 | isotropic | sample_conditions_2 |
2D 1H-15N HSQC | sample_2 | isotropic | sample_conditions_2 |
Software:
TOPSPIN v3.2 PI6, Bruker Biospin - collection, processing
CARA v1.5.5, ROCHUS KELLER - chemical shift assignment
NMR spectrometers:
- Bruker Avance HD-III HD 700 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts