BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 26802

Title: Cold Shock Protein A   PubMed: 29197185

Deposition date: 2016-05-17 Original release date: 2017-05-30

Authors: Caruso, Icaro; Panwalkar, Vineet; Arni, Raghuvir; Cornelio, Marinonio

Citation: Caruso, Icaro; Panwalkar, Vineet; Coronado, Monika; Dingley, Andrew; Cornelio, Marinonio; Willbold, Dieter; Arni, Raghuvir; Eberle, Raphael. "Structure and interaction of Corynebacterium pseudotuberculosis cold shock protein A with Y-box single-stranded DNA fragment."  FEBS J. 285, 372-390 (2018).

Assembly members:
Molecule_1, polymer, 82 residues, 9118.9276 Da.

Natural source:   Common Name: high GC Gram+   Taxonomy ID: 1719   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Corynebacterium pseudotuberculosis

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Molecule_1: MAQGTVKWFNAEKGFGFIAP DDGSADVFVHYSEIQGNGFR TLEENQKVEFEIGEGAKGPQ AQQVHALGGENLYFQGHHHH HH

Data typeCount
13C chemical shifts322
15N chemical shifts84
1H chemical shifts494

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Cold Shock Protein A1

Entities:

Entity 1, Cold Shock Protein A 82 residues - 9118.9276 Da.

1   METALAGLNGLYTHRVALLYSTRPPHEASN
2   ALAGLULYSGLYPHEGLYPHEILEALAPRO
3   ASPASPGLYSERALAASPVALPHEVALHIS
4   TYRSERGLUILEGLNGLYASNGLYPHEARG
5   THRLEUGLUGLUASNGLNLYSVALGLUPHE
6   GLUILEGLYGLUGLYALALYSGLYPROGLN
7   ALAGLNGLNVALHISALALEUGLYGLYGLU
8   ASNLEUTYRPHEGLNGLYHISHISHISHIS
9   HISHIS

Samples:

CspA: Molecule 1, [U-13C; U-15N], 0.8 mM; K2HPO4 50.0 mM; NaCl 50.0 mM; DSS 0.1 mM; H2O 90%; D2O 10%

CspA_1: ionic strength: 0.050 M; pH: 6.500; pressure: 1.000 atm; temperature: 298.000 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC/HMQCCspAisotropicCspA_1
2D 1H-13C HSQC/HMQCCspAisotropicCspA_1
hbcbcgcdhd (hbCBcgcdHD)CspAisotropicCspA_1
hbcbcgcdcehe (hbCBcgcdceHE)CspAisotropicCspA_1
3D 1H-13C NOESYCspAisotropicCspA_1
3D HNCOCspAisotropicCspA_1

Software:

CcpNmr_Analysis v2.4, CCPN - Chemical shift assignment

NMR spectrometers:

  • Varian VNMRS 900 MHz
  • Bruker Avance 599 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts