BMRB Entry 26863

Name: ScribPDZ4apo
Published: 2016-08-23

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26863

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: ScribPDZ4apo PubMed: 27694890

Deposition date: 2016-07-30 Original release date: 2016-08-23

Authors: Umitsu, Masataka; Ikura, Mitsuhiko

Citation: Zheng, Weiyue; Umitsu, Masataka; Jagan, Ishaan; Tran, Charles; Ishiyama, Noboru; BeGora, Michael; Araki, Kiyomi; Ohashi, Pamela; Ikura, Mitsuhiko; Muthuswamy, Senthil. "An interaction between Scribble and the NADPH oxidase complex controls M1 macrophage polarization and function" Nat. Cell. Biol. 18, 1244-1252 (2016).

Assembly members:
ScribPDZ4apo, polymer, 102 residues, Formula weight is not available

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
ScribPDZ4apo: GSHMPGLRELCIQKAPGERL GISIRGGARGHAGNPRDPTD EGIFISKVSPTGAAGRDGRL RVGLRLLEVNQQSLLGLTHG EAVQLLRSVGDTLTVLVCDG FE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	176
15N chemical shifts	82
1H chemical shifts	82

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ScribPDZ4apo	1

Entities:

Entity 1, ScribPDZ4apo 102 residues - Formula weight is not available

1

GLY

SER

HIS

MET

PRO

GLY

LEU

ARG

GLU

LEU

2

CYS

ILE

GLN

LYS

ALA

PRO

GLY

GLU

ARG

LEU

3

GLY

ILE

SER

ILE

ARG

GLY

ALA

ARG

GLY

4

HIS

ALA

GLY

ASN

PRO

ARG

ASP

PRO

THR

ASP

5

GLU

GLY

ILE

PHE

ILE

SER

LYS

VAL

SER

PRO

6

THR

GLY

ALA

GLY

ARG

ASP

GLY

ARG

LEU

7

ARG

VAL

GLY

LEU

ARG

LEU

GLU

VAL

ASN

8

GLN

SER

LEU

GLY

LEU

THR

HIS

GLY

9

GLU

ALA

VAL

GLN

LEU

ARG

SER

VAL

GLY

10

ASP

THR

LEU

THR

VAL

LEU

VAL

CYS

ASP

GLY

11

PHE

GLU

Samples:

sample_1_15N: ScribPDZ4apo, [U-100% 15N], 0.2 mM; NaCl 100 mM

sample_2_15N13C: ScribPDZ4apo, [U-100% 13C; U-100% 15N], 0.1 mM; NaCl 100 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1_15N	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2_15N13C	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_2_15N13C	isotropic	sample_conditions_1
3D HNCACB	sample_2_15N13C	isotropic	sample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Bruker Avance 600 MHz
Bruker Avance 800 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts