BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 30059

Title: NMR structures of hylin-a1 analogs: Hylin-D

Deposition date: 2016-04-05 Original release date: 2017-04-06

Authors: Crusca Jr., Edson; Matos, Carolina; Liao, Luciano; Oliveira, Aline

Citation: Crusca Jr., E.; Camara, A.; Matos, C.; Marchetto, R.; Cilli, E.; Liao, L.; de Oliveira,, A.L.. "NMR structures and molecular dynamics simulation of hylin-a1 analogs bound to dodecylphosphocholine micelles."  . ., .-..

Assembly members:
Hylin-D, polymer, 20 residues, 2053.513 Da.

Natural source:   Common Name: Spotted tree frog   Taxonomy ID: 279985   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Hypsiboas albopunctatus

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
Hylin-D: DIFGAIWPLALGALKNLIKX

Data sets:
Data typeCount
1H chemical shifts130

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 20 residues - 2053.513 Da.

1   ASPILEPHEGLYALAILETRPPROLEUALA
2   LEUGLYALALEULYSASNLEUILELYSNH2

Samples:

sample_1: DPC-d38, [U-100% 2H], 100 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H COSYsample_1isotropicsample_conditions_1

Software:

NMRVIEW - structure solution

X-PLOR NIH, SCHWIETERS, KUSZEWSKI, TJANDRA AND CLORE - refinement

NMR spectrometers:

  • Bruker AVANCE III 500 MHz