BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 30597

Title: Wasabi Receptor Toxin

Deposition date: 2019-03-28 Original release date: 2019-08-23

Authors: Lin King, J.; Kelly, M.; Julius, D.

Citation: Lin King, J.; Emrick, J.; Kelly, M.; Herzig, V.; King, G.; Medzihradszky, K.; Julius, D.. "A cell-penetrating scorpion toxin targets TRPA1 to elicit mode-specific activation and pain"  . ., .-..

Assembly members:
entity_1, polymer, 33 residues, 3862.348 Da.

Natural source:   Common Name: Urodacus manicatus   Taxonomy ID: 1330407   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Urodacus manicatus

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
entity_1: ASPQQAKYCYEQCNVNKVPF DQCYQMCSPLERS

Data typeCount
13C chemical shifts107
1H chemical shifts224

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 33 residues - 3862.348 Da.

1   ALASERPROGLNGLNALALYSTYRCYSTYR
2   GLUGLNCYSASNVALASNLYSVALPROPHE
3   ASPGLNCYSTYRGLNMETCYSSERPROLEU
4   GLUARGSER

Samples:

sample_1: Wasabi Receptor Toxin 300 uM

sample_2: Wasabi Receptor Toxin 300 uM

sample_conditions_1: ionic strength: 0 M; pH: 4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H COSYsample_2isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_2isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_2isotropicsample_conditions_1

Software:

ARIA, LINGE, O'DONOGHUE AND NILGES - refinement, structure calculation

CcpNmr Analysis, CCPN - chemical shift assignment, peak picking

TopSpin, Bruker Biospin - collection

PSVS, Bhattacharya and Montelione - data analysis

NMR spectrometers:

  • Bruker Avance 800 MHz