BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 34116

Title: Nuclear Magnetic Resonance Structure of the Human Polyoma JC Virus Agnoprotein   PubMed: 28295503

Deposition date: 2017-03-22 Original release date: 2017-04-20

Authors: Coric, P.; Saribas, A.; Abou-Gharbia, M.; Childers, W.; Condra, J.; White, M.; Safak, M.; Bouaziz, S.

Citation: Coric, P.; Saribas, A.; Abou-Gharbia, M.; Childers, W.; Condra, J.; White, M.; Safak, M.; Bouaziz, S.; Coric, P.; Saribas, A.; Abou-Gharbia, M.; Childers, W.; White, M.; Bouaziz, S.; Safak, M.. "Nuclear Magnetic Resonance Structure of the Human Polyoma JC Virus Agnoprotein."  J. Cell. Biochem. ., .-. (2017).

Assembly members:
entity_1, polymer, 71 residues, 8096.414 Da.

Natural source:   Common Name: JCPyV   Taxonomy ID: 10632   Superkingdom: Viruses   Kingdom: not available   Genus/species: Betapolyomavirus JCPyV

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
entity_1: MVLRQLSRKASVKVSKTWSG TKKRAQRILIFLLEFLLDFC TGEDSVDGKKRQRHSGLTEQ TYSALPEPKAT

Data sets:
Data typeCount
1H chemical shifts457

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 71 residues - 8096.414 Da.

1   METVALLEUARGGLNLEUSERARGLYSALA
2   SERVALLYSVALSERLYSTHRTRPSERGLY
3   THRLYSLYSARGALAGLNARGILELEUILE
4   PHELEULEUGLUPHELEULEUASPPHECYS
5   THRGLYGLUASPSERVALASPGLYLYSLYS
6   ARGGLNARGHISSERGLYLEUTHRGLUGLN
7   THRTYRSERALALEUPROGLUPROLYSALA
8   THR

Samples:

sample_1: agnoprotein 0.2 mM; NaCl 200 mM

sample_conditions_1: ionic strength: 200 mM; pH: 3.0; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1anisotropicsample_conditions_1
2D 1H-1H TOCSYsample_1anisotropicsample_conditions_1
2D DQF-COSYsample_1anisotropicsample_conditions_1

Software:

Analysis v2.4, CCPN - peak picking

Analysis v2.4.0, CCPN - chemical shift assignment

CNS vVersion: 1.2 at patch level 1, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure calculation

TOPSPIN v3.2, Bruker Biospin - processing

NMR spectrometers:

  • Bruker AvanceIII 600 MHz