BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 36179

Title: Solution structure of centrin4 from Trypanosoma brucei   PubMed: 30389845

Deposition date: 2018-04-15 Original release date: 2019-03-11

Authors: Shan, F.; Tu, X.

Citation: Shan, Fangzhen; Ye, Kaiqin; Zhang, Jiahai; Liao, Shanhui; Zhang, Xuecheng; Xu, Chao; Tu, Xiaoming. "Solution structure of TbCentrin4 from"  Biochem. J. 475, 3763-3778 (2018).

Assembly members:
Centrin, putative, polymer, 149 residues, 16570.627 Da.

Natural source:   Common Name: Trypanosoma brucei   Taxonomy ID: 185431   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Trypanosoma Trypanosoma brucei

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Centrin, putative: MAALTDEQIREAFNLFDADG SGAIDAEEMALAMKGLGFGD LPRDEVERTVRSMNTNANGL IEYGEFERMVKSRMAQKDSP EEVLKAFQLFDLDKKGKISF ANLKEVAKLLGENPGDDVLQ EMIAEADEDGDGEVSFEEFK SVMMQMRGK

Data sets:
Data typeCount
13C chemical shifts368
15N chemical shifts145
1H chemical shifts960

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 149 residues - 16570.627 Da.

1   METALAALALEUTHRASPGLUGLNILEARG
2   GLUALAPHEASNLEUPHEASPALAASPGLY
3   SERGLYALAILEASPALAGLUGLUMETALA
4   LEUALAMETLYSGLYLEUGLYPHEGLYASP
5   LEUPROARGASPGLUVALGLUARGTHRVAL
6   ARGSERMETASNTHRASNALAASNGLYLEU
7   ILEGLUTYRGLYGLUPHEGLUARGMETVAL
8   LYSSERARGMETALAGLNLYSASPSERPRO
9   GLUGLUVALLEULYSALAPHEGLNLEUPHE
10   ASPLEUASPLYSLYSGLYLYSILESERPHE
11   ALAASNLEULYSGLUVALALALYSLEULEU
12   GLYGLUASNPROGLYASPASPVALLEUGLN
13   GLUMETILEALAGLUALAASPGLUASPGLY
14   ASPGLYGLUVALSERPHEGLUGLUPHELYS
15   SERVALMETMETGLNMETARGGLYLYS

Samples:

sample_1: centrin4, [U-13C; U-15N], 0.5 mM; sodium chloride 200 mM; sodium phosphate 20 mM; DTT 2 mM; EDTA 2 mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 200 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert P., Guntert, Mumenthaler and Wuthrich - refinement, structure calculation

SPARKY, Goddard - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker DMX 600 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts