BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4943

Title: 1H Chemical shift Assignments for Lysozymes marketed by Seikagaku and Sigma companies at pH 3.8, 308K

Deposition date: 2001-01-19 Original release date: 2003-05-21

Authors: Poznanski, Jaroslaw; Georgalis, Yannis; Wehr, Lorin; Zielenkiewicz, Piotr; Saenger, Wolfram

Citation: Poznanski, Jaroslaw; Georgalis, Yannis; Wehr, Lorin; Zielenkiewicz, Piotr; Saenger, Wolfram. "Comparison of Two Different Lysozyme Types Under Native and Crystallization Conditions using Two-Dimensional NMR and Dynamic Light Scattering "  Biophys. Chem. ., .-. (2002).

Assembly members:
Chicken Egg White Lysozyme, polymer, 133 residues, Formula weight is not available

Natural source:   Common Name: Chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:   Production method: vendor

Entity Sequences (FASTA):
Chicken Egg White Lysozyme: KVFGRCELAAAMKRHGLDNY RGYSLGNWVCAAKFESNFNT QATNRNTDGSTDYGILQINS RWWCNDGRTPGSRNLCNIPC SALLSSDITASVNCAKKIVS DGNGMNAWVAWRNRCKGTDV QAWIRGCRLDRSR

Data sets:
Data typeCount
1H chemical shifts964

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1lysozyme1

Entities:

Entity 1, lysozyme 133 residues - Formula weight is not available

1   LYSVALPHEGLYARGCYSGLULEUALAALA
2   ALAMETLYSARGHISGLYLEUASPASNTYR
3   ARGGLYTYRSERLEUGLYASNTRPVALCYS
4   ALAALALYSPHEGLUSERASNPHEASNTHR
5   GLNALATHRASNARGASNTHRASPGLYSER
6   THRASPTYRGLYILELEUGLNILEASNSER
7   ARGTRPTRPCYSASNASPGLYARGTHRPRO
8   GLYSERARGASNLEUCYSASNILEPROCYS
9   SERALALEULEUSERSERASPILETHRALA
10   SERVALASNCYSALALYSLYSILEVALSER
11   ASPGLYASNGLYMETASNALATRPVALALA
12   TRPARGASNARGCYSLYSGLYTHRASPVAL
13   GLNALATRPILEARGGLYCYSARGLEUASP
14   ARGSERARG

Samples:

sample_A: Chicken Egg White Lysozyme 10 mM

sample_B: Chicken Egg White Lysozyme 10 mM

cond: pH: 3.8; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
1H-1H TOCSYnot availablenot availablecond
1H-1H NOESYnot availablenot availablecond

Software:

nmrpipe v1.7 - data processing

XEasy v1.3.11 - peak assignment, sequential assignment

NMR spectrometers:

  • Varian Unity Plus 500 MHz

Related Database Links:

BMRB 1093 1104 1105 11052 1650 1651 1653 1770 1772 1798 1799 1800 1801 1802 1803 18304 18305 1865 1881 1882 1883 1884 1975 1977 2231 2446 2786 2858 2917 2957 4562 4831 5068 5069 568 569 5803 5804 6415 7143 7144 7159 7160 791
PDB
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .