BMRB Entry 7370
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR7370
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Backbone 1H and 15N chemical shift assignments of D. crassirhizoma Plastocyanin PubMed: 17367139
Deposition date: 2007-02-08 Original release date: 2007-05-04
Authors: Hulsker, Rinske; Ubbink, Marcellus
Citation: Hulsker, Rinske; Mery, Annabelle; Thomassen, Ellen; Ranieri, Antonio; Sola, Marco; Verbeet, Martin; Kohzuma, Takamitsu; Ubbink, Marcellus. "Protonation of a histidine copper ligand in fern plastocyanin" J. Am. Chem. Soc. 129, 4423-4429 (2007).
Assembly members:
plastocyanin, polymer, 102 residues, Formula weight is not available
CU1, non-polymer, 63.546 Da.
Natural source: Common Name: fern Taxonomy ID: not available Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Dryopteris crassirhizoma
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
plastocyanin: AKVEVGDEVGNFKFYPDSIT
VSAGEAVEFTLVGETGHNIV
FDIPAGAPGTVASELKAASM
DENDLLSEDEPSFKAKVSTP
GTYTFYCTPHKSANMKGTLT
VK
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 95 |
| 1H chemical shifts | 95 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | plastocyanin | 1 |
| 2 | COPPER (I) ION | 2 |
Entities:
Entity 1, plastocyanin 102 residues - Formula weight is not available
| 1 | ALA | LYS | VAL | GLU | VAL | GLY | ASP | GLU | VAL | GLY | ||||
| 2 | ASN | PHE | LYS | PHE | TYR | PRO | ASP | SER | ILE | THR | ||||
| 3 | VAL | SER | ALA | GLY | GLU | ALA | VAL | GLU | PHE | THR | ||||
| 4 | LEU | VAL | GLY | GLU | THR | GLY | HIS | ASN | ILE | VAL | ||||
| 5 | PHE | ASP | ILE | PRO | ALA | GLY | ALA | PRO | GLY | THR | ||||
| 6 | VAL | ALA | SER | GLU | LEU | LYS | ALA | ALA | SER | MET | ||||
| 7 | ASP | GLU | ASN | ASP | LEU | LEU | SER | GLU | ASP | GLU | ||||
| 8 | PRO | SER | PHE | LYS | ALA | LYS | VAL | SER | THR | PRO | ||||
| 9 | GLY | THR | TYR | THR | PHE | TYR | CYS | THR | PRO | HIS | ||||
| 10 | LYS | SER | ALA | ASN | MET | LYS | GLY | THR | LEU | THR | ||||
| 11 | VAL | LYS |
Entity 2, COPPER (I) ION - Cu - 63.546 Da.
| 1 | CU1 |
Samples:
sample_1: plastocyanin, [U-15N; U-13C], 1 ± 0.05 mM; sodium phosphate 10 mM
conditions_1: ionic strength: 10 mM; pH: 6.5; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H15N_HSQC | sample_1 | not available | conditions_1 |
| HNCACB | sample_1 | not available | conditions_1 |
| HNCO | sample_1 | not available | conditions_1 |
| HN(CA)CO | sample_1 | not available | conditions_1 |
Software:
xwinnmr, Bruker - Acquisition
AZARA, freeware - Processing
Ansig-for-Windows, freeware - Assignment
NMR spectrometers:
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts