BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 10100

Title: Solution Structure of The Third PDZ Domain of Human Atrophin-1 Interacting Protein 1 (KIAA0705 Protein)

Authors: Miyamoto, K.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.

Citation: Miyamoto, K.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution Structure of The Third PDZ Domain of Human Atrophin-1 Interacting Protein 1 (KIAA0705 Protein)"  . ., .-..

Assembly members:
PDZ Domain, polymer, 103 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):
PDZ Domain: GSSGSSGYKELDVHLRRMES GFGFRILGGDEPGQPILIGA VIAMGSADRDGRLHPGDELV YVDGIPVAGKTHRYVIDLMH HAARNGQVNLTVRRKVLSGP SSG

Data sets:
Data typeCount
13C chemical shifts401
15N chemical shifts91
1H chemical shifts660

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Membrane Associated Guanylate Kinase Inverted-2 (MAGI-2)1

Entities:

Entity 1, Membrane Associated Guanylate Kinase Inverted-2 (MAGI-2) 103 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYTYRLYSGLU
2   LEUASPVALHISLEUARGARGMETGLUSER
3   GLYPHEGLYPHEARGILELEUGLYGLYASP
4   GLUPROGLYGLNPROILELEUILEGLYALA
5   VALILEALAMETGLYSERALAASPARGASP
6   GLYARGLEUHISPROGLYASPGLULEUVAL
7   TYRVALASPGLYILEPROVALALAGLYLYS
8   THRHISARGTYRVALILEASPLEUMETHIS
9   HISALAALAARGASNGLYGLNVALASNLEU
10   THRVALARGARGLYSVALLEUSERGLYPRO
11   SERSERGLY

Samples:

sample_1: PDZ domain, [U-13C; U-15N], 1.3 mM; Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1not availablecondition_1
3D 15N-separated NOESYsample_1not availablecondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.816, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - structure solution

OPALp, BILLETER, M., GUNTERT, P., KORADI, R. - refinement

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
GB EGW09165 ELK01009 EMP32129 EPY80876 ERE90474