BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 10103

Title: Solution structure of the fourth PDZ domain of human scribble (KIAA0147 protein)

Authors: Tochio, N.; Koshiba, S.; Kigawa, T.; Yokoyama, S.

Citation: Tochio, N.; Koshiba, S.; Kigawa, T.; Yokoyama, S.. "Solution structure of the fourth PDZ domain of human scribble (KIAA0147 protein)"  . ., .-..

Assembly members:
PDZ domain, polymer, 111 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):
PDZ domain: GSSGSSGPGLRELCIQKAPG ERLGISIRGGARGHAGNPRD PTDEGIFISKVSPTGAAGRD GRLRVGLRLLEVNQQSLLGL THGEAVQLLRSVGDTLTVLV CDGFESGPSSG

Data sets:
Data typeCount
13C chemical shifts425
15N chemical shifts108
1H chemical shifts696

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1KIAA0147 protein1

Entities:

Entity 1, KIAA0147 protein 111 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYPROGLYLEU
2   ARGGLULEUCYSILEGLNLYSALAPROGLY
3   GLUARGLEUGLYILESERILEARGGLYGLY
4   ALAARGGLYHISALAGLYASNPROARGASP
5   PROTHRASPGLUGLYILEPHEILESERLYS
6   VALSERPROTHRGLYALAALAGLYARGASP
7   GLYARGLEUARGVALGLYLEUARGLEULEU
8   GLUVALASNGLNGLNSERLEULEUGLYLEU
9   THRHISGLYGLUALAVALGLNLEULEUARG
10   SERVALGLYASPTHRLEUTHRVALLEUVAL
11   CYSASPGLYPHEGLUSERGLYPROSERSER
12   GLY

Samples:

sample_1: PDZ domain, [U-13C; U-15N], 1.5 mM; phosphate 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1not availablecondition_1
3D 15N-separated NOESYsample_1not availablecondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v200204025, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.816, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

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