BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 10106

Title: Solution structure of the SWIB/MDM2 domain of the hypothetical protein At5g08430 from Arabidopsis thaliana

Authors: Yoneyama, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation: Yoneyama, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the SWIB/MDM2 domain of the hypothetical protein At5g08430 from Arabidopsis thaliana"  . ., .-..

Assembly members:
SWIB/MDM2 domain, polymer, 101 residues, Formula weight is not available

Natural source:   Common Name: thale cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):
SWIB/MDM2 domain: GSSGSSGKRFEFVGWGSRQL IEFLHSLGKDTSEMISRYDV SDTIAKYISKEGLLDPSNKK KVVCDKRLVLLFGTRTIFRM KVYDLLEKHYKENQDSGPSS G

Data sets:
Data typeCount
13C chemical shifts446
15N chemical shifts99
1H chemical shifts695

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hypothetical protein RAFL09-47-K031

Entities:

Entity 1, hypothetical protein RAFL09-47-K03 101 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYLYSARGPHE
2   GLUPHEVALGLYTRPGLYSERARGGLNLEU
3   ILEGLUPHELEUHISSERLEUGLYLYSASP
4   THRSERGLUMETILESERARGTYRASPVAL
5   SERASPTHRILEALALYSTYRILESERLYS
6   GLUGLYLEULEUASPPROSERASNLYSLYS
7   LYSVALVALCYSASPLYSARGLEUVALLEU
8   LEUPHEGLYTHRARGTHRILEPHEARGMET
9   LYSVALTYRASPLEULEUGLULYSHISTYR
10   LYSGLUASNGLNASPSERGLYPROSERSER
11   GLY

Samples:

sample_1: SWIB/MDM2 domain, [U-13C; U-15N], 0.4 mM; phosphate 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1not availablecondition_1
3D 13C-separated NOESYsample_1not availablecondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Jhonson, B.A. - data analysis

Kujira v0.815, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
EMBL CAC08343
GB ACF06124 AED91300
REF NP_196460
SP Q9FT92