BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 10107

Title: Solution structure of the CH domain from mouse EB-1

Authors: Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.

Citation: Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the CH domain from mouse EB-1"  . ., .-..

Assembly members:
CH domain, polymer, 115 residues, Formula weight is not available

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):
CH domain: GSSGSSGQRRHDMLAWINES LQLNLTKIEQLCSGAAYCQF MDMLFPGSIALKKVKFQAKL EHEYIQNFKILQAGFKRMGV DKIIPVDKLVKGKFQDNFEF VQWFKKFFDSGPSSG

Data sets:
Data typeCount
13C chemical shifts539
15N chemical shifts125
1H chemical shifts834

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1microtubule-associated protein, RP/EB family, member 11

Entities:

Entity 1, microtubule-associated protein, RP/EB family, member 1 115 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYGLNARGARG
2   HISASPMETLEUALATRPILEASNGLUSER
3   LEUGLNLEUASNLEUTHRLYSILEGLUGLN
4   LEUCYSSERGLYALAALATYRCYSGLNPHE
5   METASPMETLEUPHEPROGLYSERILEALA
6   LEULYSLYSVALLYSPHEGLNALALYSLEU
7   GLUHISGLUTYRILEGLNASNPHELYSILE
8   LEUGLNALAGLYPHELYSARGMETGLYVAL
9   ASPLYSILEILEPROVALASPLYSLEUVAL
10   LYSGLYLYSPHEGLNASPASNPHEGLUPHE
11   VALGLNTRPPHELYSLYSPHEPHEASPSER
12   GLYPROSERSERGLY

Samples:

sample_1: CH domain, [U-13C; U-15N], 1.03 mM; sodium phosphate 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1not availablecondition_1
3D 15N-separated NOESYsample_1not availablecondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.863, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAG59745
REF XP_003273569 XP_004062039 XP_004325232 XP_004612629 XP_004697982