BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 10109

Title: Solution Structure of the PDZ Domain from Mouse Glutamate Receptor Interacting Protein 1A-L (GRIP1) Homolog

Authors: Sato, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation: Sato, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution Structure of the PDZ Domain from Mouse Glutamate Receptor Interacting Protein 1A-L (GRIP1) Homolog"  Not known ., .-..

Assembly members:
Glutamate Receptor Interacting Protein 1A-L Homolog, polymer, 122 residues, Formula weight is not available

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):
Glutamate Receptor Interacting Protein 1A-L Homolog: GSSGSSGAGQVVHTETTEVV LTADPVTGFGIQLQGSVFAT ETLSSPPLISYIEADSPAER CGVLQIGDRVMAINGIPTED STFEEANQLLRDSSITSKVT LEIEFDVAESVIPSSGSGPS SG

Data sets:
Data typeCount
13C chemical shifts457
15N chemical shifts111
1H chemical shifts755

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Glutamate Receptor Interacting Protein 1A-L Homolog1

Entities:

Entity 1, Glutamate Receptor Interacting Protein 1A-L Homolog 122 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYALAGLYGLN
2   VALVALHISTHRGLUTHRTHRGLUVALVAL
3   LEUTHRALAASPPROVALTHRGLYPHEGLY
4   ILEGLNLEUGLNGLYSERVALPHEALATHR
5   GLUTHRLEUSERSERPROPROLEUILESER
6   TYRILEGLUALAASPSERPROALAGLUARG
7   CYSGLYVALLEUGLNILEGLYASPARGVAL
8   METALAILEASNGLYILEPROTHRGLUASP
9   SERTHRPHEGLUGLUALAASNGLNLEULEU
10   ARGASPSERSERILETHRSERLYSVALTHR
11   LEUGLUILEGLUPHEASPVALALAGLUSER
12   VALILEPROSERSERGLYSERGLYPROSER
13   SERGLY

Samples:

sample_1: PDZ domain, [U-13C; U-15N], 0.59 mM; phosphate 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.863, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
REF XP_007506268