BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 10110

Title: Solution Structure of the Gas2 Domain of the Growth Arrest Specific 2 Protein

Authors: Miyamoto, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation: Miyamoto, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution Structure of the Gas2 Domain of the Growth Arrest Specific 2 Protein"  Not known ., .-..

Assembly members:
Growth-arrest-specific protein 2, polymer, 97 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):
Growth-arrest-specific protein 2: GSSGSSGNLLDDAVKRISED PPCKCPTKFCVERLSQGRYR VGEKILFIRMLHNKHVMVRV GGGWETFAGYLLKHDPCRML QISRVDGKTSPSGPSSG

Data sets:
Data typeCount
13C chemical shifts378
15N chemical shifts79
1H chemical shifts619

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Growth-arrest-specific protein 21
2zinc ion2

Entities:

Entity 1, Growth-arrest-specific protein 2 97 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYASNLEULEU
2   ASPASPALAVALLYSARGILESERGLUASP
3   PROPROCYSLYSCYSPROTHRLYSPHECYS
4   VALGLUARGLEUSERGLNGLYARGTYRARG
5   VALGLYGLULYSILELEUPHEILEARGMET
6   LEUHISASNLYSHISVALMETVALARGVAL
7   GLYGLYGLYTRPGLUTHRPHEALAGLYTYR
8   LEULEULYSHISASPPROCYSARGMETLEU
9   GLNILESERARGVALASPGLYLYSTHRSER
10   PROSERGLYPROSERSERGLY

Entity 2, zinc ion - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: Gas2 Domain, [U-13C; U-15N], 1.03 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 100 uM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.863, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAB29405 BAG36475 BAJ20935
EMBL CAG29281
GB AAA37660 AAC52058 AAH13456 AAH40470 AAH53446
REF NP_001098471 NP_001120976 NP_001137302 NP_001244553 NP_005247
SP O43903 P11862
TPG DAA14022