BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 10111

Title: Solution structure of the 6th HMG box of mouse UBF1

Authors: Sato, M.; Saito, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation: Sato, M.; Saito, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the 6th HMG box of mouse UBF1"  Not known ., .-..

Assembly members:
Nucleolar transcription factor 1, polymer, 101 residues, Formula weight is not available

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):
Nucleolar transcription factor 1: GSSGSSGPKKPPMNGYQKFS QELLSNGELNHLPLKERMVE IGSRWQRISQSQKEHYKKLA EEQQRQYKVHLDLWVKSLSP QDRAAYKEYISNKRKSGPSS G

Data sets:
Data typeCount
13C chemical shifts452
15N chemical shifts107
1H chemical shifts714

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Nucleolar transcription factor 11

Entities:

Entity 1, Nucleolar transcription factor 1 101 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYPROLYSLYS
2   PROPROMETASNGLYTYRGLNLYSPHESER
3   GLNGLULEULEUSERASNGLYGLULEUASN
4   HISLEUPROLEULYSGLUARGMETVALGLU
5   ILEGLYSERARGTRPGLNARGILESERGLN
6   SERGLNLYSGLUHISTYRLYSLYSLEUALA
7   GLUGLUGLNGLNARGGLNTYRLYSVALHIS
8   LEUASPLEUTRPVALLYSSERLEUSERPRO
9   GLNASPARGALAALATYRLYSGLUTYRILE
10   SERASNLYSARGLYSSERGLYPROSERSER
11   GLY

Samples:

sample_1: HMG box domain, [U-13C; U-15N], 1.2 mM; phosphate 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.816, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAE01971 BAE90957
GB ERE68360 KFO25262
REF XP_010641312 XP_537622