BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 10112

Title: Solution structure of the ubiquitin domain from mouse D7Wsu128e protein

Authors: Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.

Citation: Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the ubiquitin domain from mouse D7Wsu128e protein"  Not known ., .-..

Assembly members:
D7Wsu128e protein, polymer, 95 residues, Formula weight is not available

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):
D7Wsu128e protein: GSSGSSGDAGGGVGKELVDL KIIWNKTKHDVKVPLDSTGS ELKQKIHSITGLPPAMQKVM YKGLVPEDKTLREIKVTSGA KIMVVGSTISGPSSG

Data sets:
Data typeCount
13C chemical shifts408
15N chemical shifts93
1H chemical shifts657

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DNA segment, Chr 7, Wayne State University 128, expressed1

Entities:

Entity 1, DNA segment, Chr 7, Wayne State University 128, expressed 95 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYASPALAGLY
2   GLYGLYVALGLYLYSGLULEUVALASPLEU
3   LYSILEILETRPASNLYSTHRLYSHISASP
4   VALLYSVALPROLEUASPSERTHRGLYSER
5   GLULEULYSGLNLYSILEHISSERILETHR
6   GLYLEUPROPROALAMETGLNLYSVALMET
7   TYRLYSGLYLEUVALPROGLUASPLYSTHR
8   LEUARGGLUILELYSVALTHRSERGLYALA
9   LYSILEMETVALVALGLYSERTHRILESER
10   GLYPROSERSERGLY

Samples:

sample_1: ubiquitin domain, [U-13C; U-15N], 0.39 mM; sodium phosphate 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.863, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAB30974
GB AAH11313 EDL17262
REF NP_613055 XP_006507976 XP_006507977 XP_006507978 XP_012320157
SP Q78JW9