BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 10114

Title: Solution Structure of the C-terminal Pleckstrin Homology Domain of Sbf1 from Mouse

Authors: Li, H.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation: Li, H.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution Structure of the C-terminal Pleckstrin Homology Domain of Sbf1 from Mouse"  . ., .-..

Assembly members:
The Pleckstrin Homology Domain, polymer, 117 residues, Formula weight is not available

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):
The Pleckstrin Homology Domain: GSSGSSGRSYEGILYKKGAF MKPWKARWFVLDKTKHQLRY YDHRMDTECKGVIDLAEVEA VAPGTPTIGAPKTVDEKAFF DVKTTRRVYNFCAQDVPSAQ QWVDRIQSCLSSGPSSG

Data sets:
Data typeCount
13C chemical shifts520
15N chemical shifts118
1H chemical shifts785

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1SET binding factor 11

Entities:

Entity 1, SET binding factor 1 117 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYARGSERTYR
2   GLUGLYILELEUTYRLYSLYSGLYALAPHE
3   METLYSPROTRPLYSALAARGTRPPHEVAL
4   LEUASPLYSTHRLYSHISGLNLEUARGTYR
5   TYRASPHISARGMETASPTHRGLUCYSLYS
6   GLYVALILEASPLEUALAGLUVALGLUALA
7   VALALAPROGLYTHRPROTHRILEGLYALA
8   PROLYSTHRVALASPGLULYSALAPHEPHE
9   ASPVALLYSTHRTHRARGARGVALTYRASN
10   PHECYSALAGLNASPVALPROSERALAGLN
11   GLNTRPVALASPARGILEGLNSERCYSLEU
12   SERSERGLYPROSERSERGLY

Samples:

sample_1: Pleckstrin Homology domain, [U-13C; U-15N], 0.28 mM; PiNa 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1not availablecondition_1
3D 15N-separated NOESYsample_1not availablecondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.854, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 600 MHz

Related Database Links:

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