BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 10118

Title: 1H 13C and 15N Chemical Shift Assignments for C-terminal domain of PH0471   PubMed: 18687870

Authors: Kuwahara, Yohta; Ohno, Ayako; Morii, Taichi; Tochio, Hidehito; Shirakawa, Masahiro; Hiroaki, Hidekazu

Citation: Kuwahara, Yohta; Ohno, Ayako; Morii, Taichi; Yokoyama, Hideshi; Matsui, Ikuo; Tochio, Hidehito; Shirakawa, Masahiro; Hiroaki, Hidekazu. "The solution structure of the C-terminal domain of NfeD reveals a novel membrane-anchored OB-fold"  Protein Sci. 17, 1915-1924 (2008).

Assembly members:
PH0471 C-terminal domain, polymer, 74 residues, 8360 Da.

Natural source:   Common Name: Pyrococcus horikoshii OT3   Taxonomy ID: 70601   Superkingdom: Archaea   Kingdom: not available   Genus/species: Pyrococcus horikoshii

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
PH0471 C-terminal domain: RRETTDIGGGKYTFELKGKV GKVVKIAEDHYLVEVEGDKW IAYSDEKLSLGDRVMVVDVD GLKLKVKRIPPQLE

Data sets:
Data typeCount
13C chemical shifts270
15N chemical shifts74
1H chemical shifts552

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1PH0471_C-terminal_domain1

Entities:

Entity 1, PH0471_C-terminal_domain 74 residues - 8360 Da.

1   ARGARGGLUTHRTHRASPILEGLYGLYGLY
2   LYSTYRTHRPHEGLULEULYSGLYLYSVAL
3   GLYLYSVALVALLYSILEALAGLUASPHIS
4   TYRLEUVALGLUVALGLUGLYASPLYSTRP
5   ILEALATYRSERASPGLULYSLEUSERLEU
6   GLYASPARGVALMETVALVALASPVALASP
7   GLYLEULYSLEULYSVALLYSARGILEPRO
8   PROGLNLEUGLU

Samples:

sample_1: PH0471 C-terminal domain, [U-13C; U-15N], 0.7 mM; Na Phosphate 25 mM; NaCl 300 mM; EDTA 1 mM; H2O 95%; D2O 5%

sample_2: PH0471 C-terminal domain, [U-15N], 1 mM; Na Phosphate 25 mM; NaCl 300 mM; EDTA 1 mM; H2O 95%; D2O 5%

condition_1: ionic strength: 300 mM; pH: 5.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCsample_2not availablecondition_1
1H-13C HSQCsample_1not availablecondition_1
HNCAsample_1not availablecondition_1
HNCACBsample_1not availablecondition_1
CBCACONHsample_1not availablecondition_1
HCC(CO)NHsample_1not availablecondition_1
CC(CO)NHsample_1not availablecondition_1
HCCH-TOCSYsample_1not availablecondition_1

Software:

XWIN-NMR v3.5, Bruker - collection

NMRPipe v2.3 - collection, FT

SPARKY v3.106 - assignment

CYANA v2.0.17, PETER GUNTERT - refinement, structure solution

VNMR v6.1C, VARIAN - collection

NMR spectrometers:

  • Bruker DMX 800 MHz
  • Bruker DMX 500 MHz
  • Varian INOVA 900 MHz

Related Database Links:

PDB
DBJ BAA29559
REF WP_010884582