BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 10119

Title: DnaJ domain of human KIAA0730 protein

Authors: Kobayashi, N.; Koshiba, S.; Kigawa, T.; Yokoyama, S.

Citation: Kobayashi, N.; Koshiba, S.; Kigawa, T.; Yokoyama, S.. "Solution structure of DnaJ domain of human KIAA0730 protein"  Not known ., .-..

Assembly members:
DnaJ domain, polymer, 88 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):
DnaJ domain: MHHHHHHLVPRGSILKEVTS VVEQAWKLPESERKKIIRRL YLKWHPDKNPENHDIANEVF KHLQNEINRLEKQAFLDQNA DRASRRTF

Data sets:
Data typeCount
13C chemical shifts371
15N chemical shifts90
1H chemical shifts617

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1KIAA0730 protein1

Entities:

Entity 1, KIAA0730 protein 88 residues - Formula weight is not available

1   METHISHISHISHISHISHISLEUVALPRO
2   ARGGLYSERILELEULYSGLUVALTHRSER
3   VALVALGLUGLNALATRPLYSLEUPROGLU
4   SERGLUARGLYSLYSILEILEARGARGLEU
5   TYRLEULYSTRPHISPROASPLYSASNPRO
6   GLUASNHISASPILEALAASNGLUVALPHE
7   LYSHISLEUGLNASNGLUILEASNARGLEU
8   GLULYSGLNALAPHELEUASPGLNASNALA
9   ASPARGALASERARGARGTHRPHE

Samples:

sample_1: DnaJ domain, [U-13C; U-15N], 1.3 mM; Sodium Phosphate 20 mM; Sodium chloride 100 mM; Sodium azide 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 100 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v1.8, Delaglio, F. - processing

NMRView v5.03, Johnson - data analysis

DYANA, Guntert - structure solution

CNS v1.1, Brunger - refinement

NMR spectrometers:

  • Bruker AVANCE 600 MHz

Related Database Links:

PDB
DBJ BAA34450 BAB39340 BAD32289
GB AAF31262 AAF31263 EAX08322 EDL36107 EDL85271
REF NP_001264984 NP_055178 XP_001089964 XP_002824122 XP_003477292
SP Q9JLC8 Q9NZJ4
TPG DAA23892