BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 10120

Title: Solution structure of immunoglobulin like domain of mouse nuclear lamin

Authors: Kobayashi, N.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.

Citation: Kobayashi, N.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of immunoglobulin like domain of mouse nuclear lamin"  Not known ., .-..

Assembly members:
C-terminal immunoglobulin like domain, polymer, 151 residues, Formula weight is not available

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):
C-terminal immunoglobulin like domain: GSSGSSGQSQGGGSVTKKRK LESSESRSSFSQHARTSGRV AVEEVDEEGKFVRLRNKSNE DQSMGNWQIRRQNGDDPLMT YRFPPKFTLKAGQVVTIWAS GAGATHSPPTDLVWKAQNTW GCGSSLRTALINSTGEEVAM RKLVRSGPSSG

Data sets:
Data typeCount
13C chemical shifts568
15N chemical shifts158
1H chemical shifts922

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Lamin A1

Entities:

Entity 1, Lamin A 151 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYGLNSERGLN
2   GLYGLYGLYSERVALTHRLYSLYSARGLYS
3   LEUGLUSERSERGLUSERARGSERSERPHE
4   SERGLNHISALAARGTHRSERGLYARGVAL
5   ALAVALGLUGLUVALASPGLUGLUGLYLYS
6   PHEVALARGLEUARGASNLYSSERASNGLU
7   ASPGLNSERMETGLYASNTRPGLNILEARG
8   ARGGLNASNGLYASPASPPROLEUMETTHR
9   TYRARGPHEPROPROLYSPHETHRLEULYS
10   ALAGLYGLNVALVALTHRILETRPALASER
11   GLYALAGLYALATHRHISSERPROPROTHR
12   ASPLEUVALTRPLYSALAGLNASNTHRTRP
13   GLYCYSGLYSERSERLEUARGTHRALALEU
14   ILEASNSERTHRGLYGLUGLUVALALAMET
15   ARGLYSLEUVALARGSERGLYPROSERSER
16   GLY

Samples:

sample_1: lamin, [U-13C; U-15N], 0.88 mM; phosphate 20 mM; NaCl 100 mM; d10-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

sample_2: lamin, [U-13C; U-15N], 0.70 mM; phosphate 20 mM; NaCl 100 mM; d10-DTT 1 mM; NaN3 0.02%; D2O 100%

condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESY 1sample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESY 2sample_2isotropiccondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v2002045, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.816, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAA02476 BAA03578 BAA08569 BAA08570 BAA08571
GB AAH15302 AAH94020 ABI16251 ABI16252 EDL15275
REF NP_001002011 NP_001104572 NP_062263 XP_003502967 XP_005080182
SP P48678