BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 10126

Title: Solution structure of the R3H domain from human hypothetical protein BAA76846

Authors: Nagata, T.; Muto, Y.; Inoue, M.; Kigawa, T.; Terada, T.; Shirouzu, M.; Yokoyama, S.

Citation: Nagata, T.; Muto, Y.; Inoue, M.; Kigawa, T.; Terada, T.; Shirouzu, M.; Yokoyama, S.. "Solution structure of the R3H domain from human hypothetical protein BAA76846"  Not known ., .-..

Assembly members:
R3H domain, polymer, 124 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):
R3H domain: GSSGSSGTDSTGIDLHEFLV NTLKKNPRDRMMLLKLEQEI LEFINDNNNQFKKFPQMTSY HRMLLHRVAAYFGMDHNVDQ TGKAVIINKTSNTRIPEQRF SEHIKDEKNTEFQQRFILSG PSSG

Data sets:
Data typeCount
13C chemical shifts531
15N chemical shifts121
1H chemical shifts838

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Hypothetical KIAA1002 protein1

Entities:

Entity 1, Hypothetical KIAA1002 protein 124 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYTHRASPSER
2   THRGLYILEASPLEUHISGLUPHELEUVAL
3   ASNTHRLEULYSLYSASNPROARGASPARG
4   METMETLEULEULYSLEUGLUGLNGLUILE
5   LEUGLUPHEILEASNASPASNASNASNGLN
6   PHELYSLYSPHEPROGLNMETTHRSERTYR
7   HISARGMETLEULEUHISARGVALALAALA
8   TYRPHEGLYMETASPHISASNVALASPGLN
9   THRGLYLYSALAVALILEILEASNLYSTHR
10   SERASNTHRARGILEPROGLUGLNARGPHE
11   SERGLUHISILELYSASPGLULYSASNTHR
12   GLUPHEGLNGLNARGPHEILELEUSERGLY
13   PROSERSERGLY

Samples:

sample_1: R3H domain, [U-13C; U-15N], 1.03 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 100 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

NMRView v5.0.4, Johnson - data analysis

CYANA v2.0, Guntert, P. - refinement, structure solution

NMRPipe v2.3, Delaglio, F. - processing

Kujira v0.901, Kobayashi, N. - data analysis

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAA76846 BAC65698 BAG10408
EMBL CAH91820
GB AAH43083 AAH64442 AAI17928 AAI26609 EDL24504
REF NP_001071502 NP_001124029 NP_001126053 NP_001161764 NP_001161765
SP A0JNC2 Q80TM6 Q9Y2K5
TPG DAA29558