BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 10127

Title: Solution structure of the alpha-helical domain from mouse hypothetical PNPase

Authors: Nagata, T.; Muto, Y.; Inoue, M.; Kigawa, T.; Terada, T.; Shirouzu, M.; Yokoyama, S.

Citation: Nagata, T.; Muto, Y.; Inoue, M.; Kigawa, T.; Terada, T.; Shirouzu, M.; Yokoyama, S.. "Solution structure of the alpha-helical domain from mouse hypothetical PNPase"  Not known ., .-..

Assembly members:
alpha-helical domain, polymer, 104 residues, Formula weight is not available

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):
alpha-helical domain: GSSGSSGPQKIFTPSAEIVK YTKIIAMEKLYAVFTDYEHD KVSRDEAVNKIRLDTEEHLK EKFPEVDQFEIIESFNIVAK EVFRSIILNEYKRCDGRDSG PSSG

Data sets:
Data typeCount
13C chemical shifts427
15N chemical shifts88
1H chemical shifts676

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hypothetical PNPase1

Entities:

Entity 1, hypothetical PNPase 104 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYPROGLNLYS
2   ILEPHETHRPROSERALAGLUILEVALLYS
3   TYRTHRLYSILEILEALAMETGLULYSLEU
4   TYRALAVALPHETHRASPTYRGLUHISASP
5   LYSVALSERARGASPGLUALAVALASNLYS
6   ILEARGLEUASPTHRGLUGLUHISLEULYS
7   GLULYSPHEPROGLUVALASPGLNPHEGLU
8   ILEILEGLUSERPHEASNILEVALALALYS
9   GLUVALPHEARGSERILEILELEUASNGLU
10   TYRLYSARGCYSASPGLYARGASPSERGLY
11   PROSERSERGLY

Samples:

sample_1: alpha-helical domain, [U-13C; U-15N], 1.12 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 100 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

NMRView v5.0.4, Johnson - data analysis

CYANA v2.0, Guntert, P. - refinement, structure solution

NMRPipe v2.3, Delaglio, F. - processing

Kujira v0.901, Kobayashi, N. - data analysis

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAB23374 BAE25732 BAE28862 BAE36595 BAE38260
EMBL CAD45436
GB AAH27228 AAH55826 EDL23813 EDL23814
REF NP_082145 XP_006514876 XP_011242053
SP Q8K1R3