BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 10128

Title: Solution structure of the second FNIII domain of DSCAML1 protein

Authors: Qin, X.; Hayashi, F.; Yokoyama, S.

Citation: Qin, X.; Hayashi, F.; Yokoyama, S.. "Solution structure of the second FNIII domain of DSCAML1 protein"  Not known ., .-..

Assembly members:
FNIII domain, polymer, 122 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):
FNIII domain: GSSGSSGISTEEAAPDGPPM DVTLQPVTSQSIQVTWKAPK KELQNGVIRGYQIGYRENSP GSNGQYSIVEMKATGDSEVY TLDNLKKFAQYGVVVQAFNR AGTGPSSSEINATTLESGPS SG

Data sets:
Data typeCount
13C chemical shifts503
15N chemical shifts130
1H chemical shifts799

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1second FNIII domain of DSCAML1 protein1

Entities:

Entity 1, second FNIII domain of DSCAML1 protein 122 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYILESERTHR
2   GLUGLUALAALAPROASPGLYPROPROMET
3   ASPVALTHRLEUGLNPROVALTHRSERGLN
4   SERILEGLNVALTHRTRPLYSALAPROLYS
5   LYSGLULEUGLNASNGLYVALILEARGGLY
6   TYRGLNILEGLYTYRARGGLUASNSERPRO
7   GLYSERASNGLYGLNTYRSERILEVALGLU
8   METLYSALATHRGLYASPSERGLUVALTYR
9   THRLEUASPASNLEULYSLYSPHEALAGLN
10   TYRGLYVALVALVALGLNALAPHEASNARG
11   ALAGLYTHRGLYPROSERSERSERGLUILE
12   ASNALATHRTHRLEUGLUSERGLYPROSER
13   SERGLY

Samples:

sample_1: FNIII domain, [U-13C; U-15N], 1.05 mM; PiNa 20 mM; NaCl 100 mM; d10-DTT 1.0 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.854, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

PDB
GB EDL25653
REF XP_008567853 XP_009635232 XP_009915740 XP_010117466 XP_010180789