BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 10131

Title: Solution Structure of the N-terminal Pleckstrin Homology Domain Of TAPP2 from Mouse

Authors: Li, H.; Hayashi, F.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation: Li, H.; Hayashi, F.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution Structure of the N-terminal Pleckstrin Homology Domain Of TAPP2 from Mouse"  Not known ., .-..

Assembly members:
Pleckstrin Homology domain, polymer, 126 residues, Formula weight is not available

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):
Pleckstrin Homology domain: GSSGSSGMPYVDRQNRICGF LDIEDNENSGKFLRRYFILD TQANCLLWYMDNPQNLAVGA GAVGSLQLTYISKVSIATPK QKPKTPFCFVINALSQRYFL QANDQKDLKDWVEALNQASK SGPSSG

Data sets:
Data typeCount
13C chemical shifts537
15N chemical shifts135
1H chemical shifts847

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1pleckstrin homology domain-containing, family A1

Entities:

Entity 1, pleckstrin homology domain-containing, family A 126 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYMETPROTYR
2   VALASPARGGLNASNARGILECYSGLYPHE
3   LEUASPILEGLUASPASNGLUASNSERGLY
4   LYSPHELEUARGARGTYRPHEILELEUASP
5   THRGLNALAASNCYSLEULEUTRPTYRMET
6   ASPASNPROGLNASNLEUALAVALGLYALA
7   GLYALAVALGLYSERLEUGLNLEUTHRTYR
8   ILESERLYSVALSERILEALATHRPROLYS
9   GLNLYSPROLYSTHRPROPHECYSPHEVAL
10   ILEASNALALEUSERGLNARGTYRPHELEU
11   GLNALAASNASPGLNLYSASPLEULYSASP
12   TRPVALGLUALALEUASNGLNALASERLYS
13   SERGLYPROSERSERGLY

Samples:

sample_1: Pleckstrin Homology domain, [U-13C; U-15N], 1.08 mM; PiNa 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

VNMR v6.1c, Varian - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.854, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Varian INOVA 900 MHz

Related Database Links:

PDB
DBJ BAC37176 BAC40325
GB AAG15198 AAH10215 AAL57436 EDL32841 EDL32843
REF NP_112547 XP_001071937 XP_002720821 XP_003412568 XP_003751669
SP Q9ERS5