BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 10132

Title: 1H and 15N assignment of oxidized Pseudomonas aeruginosa cytochrome c551   PubMed: 17658890

Authors: Takayama, Shin-ichi; Takahashi, Yo-ta; Mikami, Shin-ichi; Irie, Kiyofumi; Kawano, Shin; Yamamoto, Yasuhiko; Hemmi, Hikaru; Kitahara, Ryo; Yokoyama, Shigeyuki; Akasaka, Kazuyuki

Citation: Takayama, Shin-ichi; Takahashi, Yo-ta; Mikami, Shin-ichi; Irie, Kiyofumi; Kawano, Shin; Yamamoto, Yasuhiko; Hemmi, Hikaru; Kitahara, Ryo; Yokoyama, Shigeyuki; Akasaka, Kazuyuki. "Local conformational transition of Hydrogenobacter thermophilus cytochrome c552 relevant to its redox potential."  Biochemistry 46, 9215-9224 (2007).

Assembly members:
cytochrome c551, polymer, 82 residues, Formula weight is not available
HEC, non-polymer, 618.503 Da.

Natural source:   Common Name: Pseudomonas aeruginosa   Taxonomy ID: 287   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Pseudomonas aeruginosa

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
cytochrome c551: EDPEVLFKNKGCVACHAIDT KMVGPAYKDVAAKFAGQAGA EAELAQRIKNGSQGVWGPIP MPPNAVSDDEAQTLAKWVLS QK

Data sets:
Data typeCount
15N chemical shifts74
1H chemical shifts74

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1cytochrome c1
2heme2

Entities:

Entity 1, cytochrome c 82 residues - Formula weight is not available

1   GLUASPPROGLUVALLEUPHELYSASNLYS
2   GLYCYSVALALACYSHISALAILEASPTHR
3   LYSMETVALGLYPROALATYRLYSASPVAL
4   ALAALALYSPHEALAGLYGLNALAGLYALA
5   GLUALAGLULEUALAGLNARGILELYSASN
6   GLYSERGLNGLYVALTRPGLYPROILEPRO
7   METPROPROASNALAVALSERASPASPGLU
8   ALAGLNTHRLEUALALYSTRPVALLEUSER
9   GLNLYS

Entity 2, heme - C34 H34 Fe N4 O4 - 618.503 Da.

1   HEC

Samples:

sample_1: cytochrome c551, [U-15N], 1 mM; potassium phosphate 20 mM; potassium ferricyanide 10 mM

Cond_1: pH: 6.0; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
1H15N HSQCsample_1not availableCond_1

Software:

No software information available

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 10133 1333 1334 1734 1866 2968
PDB
DBJ BAD11780 BAK92489 BAP19663 BAP48532 BAQ37290
EMBL CAA35703 CAA35958 CAW25242 CCQ85614 CDH68848
GB AAG03907 AAT49429 ABJ15480 ABR83995 AEO73006
PRF 630481A
REF NP_249209 WP_003084872 WP_003118863 WP_003129143 WP_012074083
SP P00099