BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 10136

Title: Solution structure of the first SH3 domain of human intersectin2 (KIAA1256)

Authors: Kobayashi, N.; Kigawa, T.; Koshiba, S.; Tochio, N.; Inoue, M.; Yokoyama, S.

Citation: Kobayashi, N.; Kigawa, T.; Koshiba, S.; Tochio, N.; Inoue, M.; Yokoyama, S.. "Solution structure of the first SH3 domain of human intersectin2 (KIAA1256)"  Not known ., .-..

Assembly members:
Intersectin 2, polymer, 93 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):
Intersectin 2: GSSGSSGYRALYPFEARNHD EMSFNSGDIIQVDEKTVGEP GWLYGSFQGNFGWFPCNYVE KMPSSENEKAVSPKKALLPP TVSLSATSGPSSG

Data sets:
Data typeCount
13C chemical shifts392
15N chemical shifts92
1H chemical shifts576

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Intersectin 21

Entities:

Entity 1, Intersectin 2 93 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYTYRARGALA
2   LEUTYRPROPHEGLUALAARGASNHISASP
3   GLUMETSERPHEASNSERGLYASPILEILE
4   GLNVALASPGLULYSTHRVALGLYGLUPRO
5   GLYTRPLEUTYRGLYSERPHEGLNGLYASN
6   PHEGLYTRPPHEPROCYSASNTYRVALGLU
7   LYSMETPROSERSERGLUASNGLULYSALA
8   VALSERPROLYSLYSALALEULEUPROPRO
9   THRVALSERLEUSERALATHRSERGLYPRO
10   SERSERGLY

Samples:

sample_1: SH3 domain, [U-13C; U-15N], 0.54 mM; phosphate 20 mM; NaCl 100 mM; d10-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.816, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

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