BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 10138

Title: Solution structure of the N-terminal domain of RimM from Thermus thermophilus HB8   PubMed: 17616598

Authors: Suzuki, S.; Matsumoto, E.; Tatsuguchi, A.; Kawazoe, M.; Kaminishi, T.; Takemoto, C.; Shirouzu, M.; Muto, Y.; Yokoyama, S.

Citation: Suzuki, S.; Tatsuguchi, A.; Matsumoto, E.; Kawazoe, M.; Kaminishi, T.; Shirouzu, M.; Muto, Y.; Takemoto, C.; Yokoyama, S.. "Structural characterization of the ribosome maturation protein, RimM"  J. Bacteriol. 189, .-6397 (6406).

Assembly members:
RIEF fold, polymer, 85 residues, Formula weight is not available

Natural source:   Common Name: Thermus thermophilus   Taxonomy ID: 300852   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Thermus thermophilus

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):
RIEF fold: MRLVEIGRFGAPYALKGGLR FRGEPVVLHLERVYVEGHGW RAIEDLYRVGEELVVHLAGV TDRTLAEALVGLRVYAEVAD LPPLE

Data sets:
Data typeCount
13C chemical shifts388
15N chemical shifts82
1H chemical shifts623

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Probable 16S rRNA-processing protein rimM1

Entities:

Entity 1, Probable 16S rRNA-processing protein rimM 85 residues - Formula weight is not available

1   METARGLEUVALGLUILEGLYARGPHEGLY
2   ALAPROTYRALALEULYSGLYGLYLEUARG
3   PHEARGGLYGLUPROVALVALLEUHISLEU
4   GLUARGVALTYRVALGLUGLYHISGLYTRP
5   ARGALAILEGLUASPLEUTYRARGVALGLY
6   GLUGLULEUVALVALHISLEUALAGLYVAL
7   THRASPARGTHRLEUALAGLUALALEUVAL
8   GLYLEUARGVALTYRALAGLUVALALAASP
9   LEUPROPROLEUGLU

Samples:

sample_1: RIEF fold, [U-13C; U-15N], 1.2 mM; sodium phosphate 20 mM; D2O 10%; H2O 90%

condition_1: ionic strength: 120 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1not availablecondition_1
3D 13C-separated NOESYsample_1not availablecondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9321, Kobayashi, N. - data analysis

Olivia v1.10.5, Yokochi, M. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

SPARKY v3.110, Goddard, T.D., Kneller, D.G. - data analysis

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

BMRB 10139 10140
PDB
DBJ BAD70856
GB AAS81019 AEG33454 AFH38925 EIA39096
REF WP_008632437 WP_011228392 YP_144299
SP Q5SJH5 Q72JU7