BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 10142

Title: Solution structure of the N-terminal extended 20th Filamin domain from human Filamin-B

Authors: Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.

Citation: Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structure of the N-terminal extended 20th Filamin domain from human Filamin-B"  Not known ., .-..

Assembly members:
filamin domain, polymer, 106 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):
filamin domain: GSSGSSGRVKESITRTSRAP SVATVGSICDLNLKIPEINS SDMSAHVTSPSGRVTEAEIV PMGKNSHCVRFVPQEMGVHT VSVKYRGQHVTGSPFQFTVG PLGEGG

Data sets:
Data typeCount
13C chemical shifts427
15N chemical shifts96
1H chemical shifts670

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Filamin-B1

Entities:

Entity 1, Filamin-B 106 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYARGVALLYS
2   GLUSERILETHRARGTHRSERARGALAPRO
3   SERVALALATHRVALGLYSERILECYSASP
4   LEUASNLEULYSILEPROGLUILEASNSER
5   SERASPMETSERALAHISVALTHRSERPRO
6   SERGLYARGVALTHRGLUALAGLUILEVAL
7   PROMETGLYLYSASNSERHISCYSVALARG
8   PHEVALPROGLNGLUMETGLYVALHISTHR
9   VALSERVALLYSTYRARGGLYGLNHISVAL
10   THRGLYSERPROPHEGLNPHETHRVALGLY
11   PROLEUGLYGLUGLYGLY

Samples:

sample_1: filamin domain, [U-13C; U-15N], 1.53 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1not availablecondition_1
3D 15N-separated NOESYsample_1not availablecondition_1

Software:

TOPSPIN v1.3, Bruker - collection

NMRPipe v20060524, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9747, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE II 900 MHz

Related Database Links:

PDB
GB AAL68442 AAL68443
REF XP_006040270 XP_006040272