BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 10147

Title: Solution structure of the CH domain from human Sperm flagellar protein 1

Authors: Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.

Citation: Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structure of the CH domain from human Sperm flagellar protein 1"  Not known ., .-..

Assembly members:
CH domain, polymer, 127 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):
CH domain: GSSGSSGMASSVDEEALHQL YLWVDNIPLSRPKRNLSRDF SDGVLVAEVIKFYFPKMVEM HNYVPANSLQQKLSNWGHLN RKVLKRLNFSVPDDVMRKIA QCAPGVVELVLIPLRQRLEE RQRRRKQ

Data sets:
Data typeCount
13C chemical shifts557
15N chemical shifts124
1H chemical shifts914

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Sperm flagellar protein 11

Entities:

Entity 1, Sperm flagellar protein 1 127 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYMETALASER
2   SERVALASPGLUGLUALALEUHISGLNLEU
3   TYRLEUTRPVALASPASNILEPROLEUSER
4   ARGPROLYSARGASNLEUSERARGASPPHE
5   SERASPGLYVALLEUVALALAGLUVALILE
6   LYSPHETYRPHEPROLYSMETVALGLUMET
7   HISASNTYRVALPROALAASNSERLEUGLN
8   GLNLYSLEUSERASNTRPGLYHISLEUASN
9   ARGLYSVALLEULYSARGLEUASNPHESER
10   VALPROASPASPVALMETARGLYSILEALA
11   GLNCYSALAPROGLYVALVALGLULEUVAL
12   LEUILEPROLEUARGGLNARGLEUGLUGLU
13   ARGGLNARGARGARGLYSGLN

Samples:

sample_1: CH domain, [U-13C; U-15N], 1.22 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1not availablecondition_1
3D 15N-separated NOESYsample_1not availablecondition_1

Software:

TOPSPIN v1.3, Bruker - collection

NMRPipe v20060524, Delaglio, F - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9823, Kobayashi, N. - data analysis

CYANA v2.0.17, Guentert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE II 900 MHz

Related Database Links:

PDB
DBJ BAG36504
EMBL CAB45745
GB AAH22476 ABQ59055 ADZ15948 AIC59905 EAX10503
REF NP_001181610 NP_056232 XP_001161874 XP_003821360 XP_003905065
SP Q9Y4P9