BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 10148

Title: Solution structure of three zf-C2H2 domains from mouse protein odd-skipped-related 2 splicing isoform 2

Authors: Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.

Citation: Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structure of three zf-C2H2 domains from mouse protein odd-skipped-related 2 splicing isoform 2"  Not known ., .-..

Assembly members:
zf-C2H2 domains, polymer, 106 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):
zf-C2H2 domains: GSSGSSGGRLPSKTKKEFIC KFCGRHFTKSYNLLIHERTH TDERPYTCDICHKAFRRQDH LRDHRYIHSKEKPFKCQECG KGFCQSRTLAVHKTLHMQTS SPTAAS

Data sets:
Data typeCount
13C chemical shifts456
15N chemical shifts89
1H chemical shifts679

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1zf-C2H2 domains1
2ZINC ION No.12
3ZINC ION No.22
4ZINC ION No.32

Entities:

Entity 1, zf-C2H2 domains 106 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYGLYARGLEU
2   PROSERLYSTHRLYSLYSGLUPHEILECYS
3   LYSPHECYSGLYARGHISPHETHRLYSSER
4   TYRASNLEULEUILEHISGLUARGTHRHIS
5   THRASPGLUARGPROTYRTHRCYSASPILE
6   CYSHISLYSALAPHEARGARGGLNASPHIS
7   LEUARGASPHISARGTYRILEHISSERLYS
8   GLULYSPROPHELYSCYSGLNGLUCYSGLY
9   LYSGLYPHECYSGLNSERARGTHRLEUALA
10   VALHISLYSTHRLEUHISMETGLNTHRSER
11   SERPROTHRALAALASER

Entity 2, ZINC ION No.1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: zf-C2H2 domains, [U-13C; U-15N], 0.86 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1not availablecondition_1
3D 15N-separated NOESYsample_1not availablecondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20060524, Delaglio, F - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9823, Kobayashi, N. - data analysis

CYANA v2.0.17, Guentert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 700 MHz

Related Database Links:

PDB
DBJ BAC34859 BAF84180
GB AAH13504 AAH16936 AAH79211 AAI02144 AAI08580
REF NP_001012118 NP_001029500 NP_001089872 NP_001090353 NP_001163815
SP Q0IHB8 Q32NK7 Q3T135 Q6AY34
TPG DAA22584