BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 10149

Title: Solution structure of the CH domain from human MICAL-2

Authors: Tomizawa, T.; Tochio, N.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.

Citation: Tomizawa, T.; Tochio, N.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structure of the CH domain from human MICAL-2"  . ., .-..

Assembly members:
CH domain, polymer, 121 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):
CH domain: GSSGSSGDIRPSKLLTWCQQ QTEGYQHVNVTDLTTSWRSG LALCAIIHRFRPELINFDSL NEDDAVENNQLAFDVAEREF GIPPVTTGKEMASAQEPDKL SMVMYLSKFYELFRGTPLRP V

Data sets:
Data typeCount
13C chemical shifts529
15N chemical shifts123
1H chemical shifts835

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Protein MICAL-21

Entities:

Entity 1, Protein MICAL-2 121 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYASPILEARG
2   PROSERLYSLEULEUTHRTRPCYSGLNGLN
3   GLNTHRGLUGLYTYRGLNHISVALASNVAL
4   THRASPLEUTHRTHRSERTRPARGSERGLY
5   LEUALALEUCYSALAILEILEHISARGPHE
6   ARGPROGLULEUILEASNPHEASPSERLEU
7   ASNGLUASPASPALAVALGLUASNASNGLN
8   LEUALAPHEASPVALALAGLUARGGLUPHE
9   GLYILEPROPROVALTHRTHRGLYLYSGLU
10   METALASERALAGLNGLUPROASPLYSLEU
11   SERMETVALMETTYRLEUSERLYSPHETYR
12   GLULEUPHEARGGLYTHRPROLEUARGPRO
13   VAL

Samples:

sample_1: CH domain, [U-13C; U-15N], 1.20 mM; d-Tris-HCl(pH 7.0) 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1not availablecondition_1
3D 15N-separated NOESYsample_1not availablecondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20060524, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9747, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 900 MHz

Related Database Links:

PDB
GB EFB30164
REF XP_003919889 XP_004394651 XP_004394652 XP_004394653 XP_004394654